Andres Stucky

5 Questions 4 Answers 0 Followers

Questions related from Andres Stucky

My miniblot transfer system is overheating and proteins are not transferring ?

I recently bought a western mini blot system from invitrogen, The gel runs fine but when I try to transfer it the sponge pads and filter paper get really hot but the proteins don't actually...

07 July 2018 9,220 0 View

How many cells do I need to harvest to run a western blot?

I need to run a western blot of some mesenchymal stem cells and was wondering how many cells I need to run a to run a western blot Thank you

03 March 2018 1,153 4 View

Is there a specific DNA sequence that could be used to distinguish between HPV 16 and HPV 18 ?

I have some sequencing data from patients with cervical cancer and we have been able to determine that a good number of them, but we are having trouble telling for sure between hpv 16 and hpv 18....

10 October 2017 4,159 4 View

Is there a human cell line that is is infected with hpv-16 and both the original and the infected cell lines are available. Thank you

I am interested in studying the gene expression changes associated with hpv-16 in an human cell line but would like to have a control cell line as close as possible to the infected cell. I know...

05 May 2017 2,054 4 View

Can I use a PCR product with a 0.5 260/230 ratio for sequencing?

Hello everyone I am running a pcr of some genomic DNA and it seems to amplify the genes I looking for fine however when I check the sample in the nanodrop I get a curve like in the picture 260/280...

02 February 2015 3,447 5 View