Can you please suggest a most reliable protocol for Cellulase zymography?

I am using Bacillus subtilis as a source for cellulase production grown on 0.1% CMC (Carboxy-Methyl-Cellulose) media. And now I want to detect the presence of cellulase using 0.1% congo red stain...

02 March 2015 7,909 7 View

Do I need to add PMSF (Serine protease inhibitor) separately even after adding the protease inhibitor cocktail?

I am isolating proteins from yeasts.

01 February 2015 10,002 12 View

What is the maximum time period for fluorescence of DCF remains?

I am using carboxy-H2DCFDA to detect ROS in Saccharomyces cerevisiae. I will load the dye before incubating my cells with the desired agonist. What is the desired time period till when I can...

01 February 2015 4,008 3 View

I want to do a structural comparison of yeast and human metallothionein using bioinformatics tools. Can anybody suggest a suitable tool?

I want to analyse whether a human metallothionein standard for HPLC can be used for yeast metallothionein as well.

31 December 2014 7,516 2 View

Can anyone suggest to me a protocol to measure Metallothionein concentrations in yeast cells using HPLC?

I want to analyse the metallothionein expression level due to affect of heavy metals as a standardization protocol so that it can be extrapolated to mammalian cultures.

09 October 2014 3,492 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View