Tarana Arman

6 Questions 5 Answers 0 Followers

Questions related from Tarana Arman

Is there a replacement for succinate buffer (pH-5.8) containing 10mM DTT in cellulase zymography?

I am doing a cellulase isolation and purification from Bacillus sp. I have done the plate assay, and have got about 12 positive results from it, one of it is Bacillus subtilis. I want succinate...

03 March 2015 1,572 1 View

Can you please suggest a most reliable protocol for Cellulase zymography?

I am using Bacillus subtilis as a source for cellulase production grown on 0.1% CMC (Carboxy-Methyl-Cellulose) media. And now I want to detect the presence of cellulase using 0.1% congo red stain...

03 March 2015 7,964 7 View

Do I need to add PMSF (Serine protease inhibitor) separately even after adding the protease inhibitor cocktail?

I am isolating proteins from yeasts.

02 February 2015 10,035 12 View

What is the maximum time period for fluorescence of DCF remains?

I am using carboxy-H2DCFDA to detect ROS in Saccharomyces cerevisiae. I will load the dye before incubating my cells with the desired agonist. What is the desired time period till when I can...

02 February 2015 4,064 3 View

I want to do a structural comparison of yeast and human metallothionein using bioinformatics tools. Can anybody suggest a suitable tool?

I want to analyse whether a human metallothionein standard for HPLC can be used for yeast metallothionein as well.

01 January 2015 7,543 2 View

Can anyone suggest to me a protocol to measure Metallothionein concentrations in yeast cells using HPLC?

I want to analyse the metallothionein expression level due to affect of heavy metals as a standardization protocol so that it can be extrapolated to mammalian cultures.

10 October 2014 3,517 3 View