Is there a way to trace recent cell to cell contact (ie whether a cell has recently contacted another cell?)
Dear Jode,
Methods Mol Biol. 2012;827:143-55. doi: 10.1007/978-1-61779-442-1_10.
Quantitative and robust assay to measure cell-cell contact assembly and maintenance.
Nola S1, Erasmus JC, Braga VM.
Best
Dear Jode,
Methods Mol Biol. 2012;827:143-55. doi: 10.1007/978-1-61779-442-1_10.
Quantitative and robust assay to measure cell-cell contact assembly and maintenance.
Nola S1, Erasmus JC, Braga VM.
Best
Formation of mature cell adhesion requires the binding of E-cadherin with actin cytoskeleton and correlates with a resistance of cadherins to extraction by non-ionic detergents such as Triton X-100 (Adams CL, Nelson WJ, Smith SJ. Quantitative analysis of cadherin-catenin actin reorganization during development of cell-cell adhesion. J Cell Biol 1996;135:1899-911).
E-cadherin TX-100 insoluble fraction is associated with the cytoskeleton and involved into mature adhesive complexes (Hinck L, Nathke IS, Papkoff J, Nelson WJ. Dynamics of cadherin/catenin complex formation: novel protein interactions and pathways of complex assembly. J Cell Biol 1994;125:1327-40; McNeill H, Ryan TA, Smith SJ, Nelson WJ. Spatial and temporal dissection of immediate and early events following cadherin-mediated epithelial cell adhesion. J Cell Biol 1993;120:1217-26.).
To evaluate the state of the E-cadherin dependent cell adhesion cells growing on chamber slides were first extracted with 0.2% Triton X-100 buffer and then fixed. After that cells were labeled with anti-E-cadherin antibodies. If cells have developed E-cadherin-dependent adhesion, E-cadherin will be detected on the cell membrane at the site of cell-cell contacts (Immunofluorescence microscopy). (Definition of a direct extracellular interaction between Met and E-cadherin. Galina Reshetnikov, Sergei Troyanovsky, David L. Rimm. Cell Biology International 31 (2007) 366-373.)
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