Is there a way to conjugate 2 antibodies by the Fc regions, in a sililar way as Miltenyis IFNg secretion assay?

The IFNg secretion assay uses 2 antibodies (one surface marker and one interferong specific) that are conjugated together. Is it possible to take 2 urified antibodies and do the same conjugation...

01 February 2016 7,161 6 View

Does anyone know the HLA type for the NK cell line NKL?

The HLA type for NKL

10 November 2015 4,580 0 View

Is there a method for detecting recent cell-to-cell contacts?

Is there a way to trace recent cell to cell contact (ie whether a cell has recently contacted another cell?)

06 July 2015 8,664 4 View

Is there a reversible way to induce quiescence in immune cells?

I would like to induce quiesence in a cell line and trace recovery. Is there an effective way to do this?

06 July 2015 6,402 5 View

Is it possible to label a soluble ligand to irreversibly attach to a surface receptor upon binding?

To identify a cell surface target, is it possible to capture it using irreversible binding to its ligand? Don't recall the precise technology.

04 May 2014 678 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

No title

03 March 2021 5,360 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View