Is there a genetic promoter system which is transactivated by something biologically and environmentally inert?

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Can I directly ligate Illumina adaptor to the end of ssDNA?

I have a sequence of ssDNA that I would like to directly ligate an Illumina sequencing adaptor to. As long as I have done the end repair (remove phosphates), can I directly ligate the adaptor to...

26 June 2024 4,522 0 View

How to prevent Vibratome Section shrinkage after 14 day culture?

I cut 50 um sections of tissue in agarose and need to culture them for 14 days before fixing.The sections have great surface area when I first section them, but after 14 days they shrink to sizes...

28 May 2024 9,861 0 View

Can 100% alcohol (95% EtOH, 5% methanol/isopropyl alcohol) be used for bacterial SEM dehydration?

I am preparing a protocol for SEM of a biofilm, but do not have easy access to 100% ethanol for the dehydration steps. Can 100% ethanol be substituted for 100% alcohol (95% EtOH, 5%...

07 May 2024 1,515 3 View

Crab identification via AI or other automated approaches?

I was curious if anyone has used AI or other software for identifying a species (particularly a crustacean or crab) automatically? I am reviewing monitoring approaches for the European green crab,...

18 April 2024 7,408 2 View

A good microscope for identification and pictures of aquatic larvae?

I am looking to get a microscope for the identification of larvae in aquatic invasive species work. Would like to use it for the identification of larvae for aquatic invasive species work....

18 April 2024 4,401 2 View

Can a single logical model incorporate objects that appear as things/objects at one scale, but relations at another?

Hello,o o o o o o o o o I have master's level training in logic and meta-logic, high marks, A's, but am not a practicing logician at all, I am a neo-empiricist pluralist (epistemic and...

18 April 2024 9,669 4 View

Buffering pH of DNA Binding Solutions for Silica Columns?

I am trying to prepare some custom DNA binding solutions for purifying DNA with silica columns. I have prepared one with 4 M guanidine thiocyanate, 30% isopropanol, and 10 mM Phosphate buffer, pH...

03 December 2023 2,197 0 View

How to convert precipitation rate from kilogram per square meter per second (kg m-2 s-1) to millimeter per hour (mm h-1)?

Hello! I need some guidance or clarification regarding the units for precipitation rate or precipitation intensity (esp. for rainfall). I'm used to precipitation intensity being expressed in...

26 October 2023 9,704 2 View

How do I layer Cells on one another?

Hi, I have extensive 2D and 3D spheroid cell culture experience and I am considering a multi-cellular culture whereby I layer cell types on top of each other, for example stromal fibroblasts...

23 October 2023 2,131 2 View

Is this a Foraminifer?

I want to ask you if this is a foram or not. If it is, what genre or even specie we're talking about? Please. This is a sample collected in the East Pacific Ocean, specifically in the coast of...

18 September 2023 6,667 4 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View