Can anyone suggest antibody against catalytic domain of human DNMT3A (amino acids P602-V912)?

Can anyone suggest antibody (suitable for WB, IF) against catalytic domain of human DNMT3A (amino acids P602-V912)? I went through several antibodies, but they were generated using synthetic...

03 December 2020 8,670 2 View

Simple 16S PCR-based fingerprinting method to identify bacterial diversity in stocks?

I am looking for a very simple approach to identify bacterial diversity in bacterial cultures. I have unselectively cultivated fecal samples based on a dilution series, so that only one single...

16 March 2020 1,051 4 View

Can someone help to identify a representative sample size for a study that uses Fisher Exact?

Hello, can someone please help to identify a representative sample for my study, where I am using Fisher Exact to examine the significance of the association between two categorical variables? The...

18 March 2019 5,065 4 View

What software do you use to manage animal facility?

Hello, I am looking for tips for software to maintain some system in records from our animal facility. I am looking for something where I can put in information about mice (age, gender, strain,...

12 September 2018 1,273 1 View

Which are the best indices for measuring species diversity?

28 October 2017 3,005 43 View

Does anyone know why I get different results for ANOSIM in Qiime and R?

Hello everyone, I have a 16S rRNA dataset of the gut microbiome of 11 humans, who were given two different dietary interventions. For beta diversity analysis I did ANOSIM of the Bray Curtis...

05 March 2017 8,800 2 View

Do you have some experiences with extraction of miRNAs from CSF?

11 July 2016 8,407 3 View

How do I combine data sets from miRNA target gene prediction tools, i.e. TargetScan, PicTar, Diana?

I'm trying to identify miRNA targets using online tools such as Targetscan, Pictar, Diana, miRDB. I am trying to find a final target list (intersect of all sub lists) in order to make...

12 June 2014 4,314 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

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17 February 2021 4,217 3 View

Cyclooctatraene damaged by TFA?

16 February 2021 4,747 3 View