Can anyone suggest antibody against catalytic domain of human DNMT3A (amino acids P602-V912)?

Can anyone suggest antibody (suitable for WB, IF) against catalytic domain of human DNMT3A (amino acids P602-V912)? I went through several antibodies, but they were generated using synthetic...

03 December 2020 8,670 2 View

Simple 16S PCR-based fingerprinting method to identify bacterial diversity in stocks?

I am looking for a very simple approach to identify bacterial diversity in bacterial cultures. I have unselectively cultivated fecal samples based on a dilution series, so that only one single...

16 March 2020 1,051 4 View

Can someone help to identify a representative sample size for a study that uses Fisher Exact?

Hello, can someone please help to identify a representative sample for my study, where I am using Fisher Exact to examine the significance of the association between two categorical variables? The...

18 March 2019 5,065 4 View

Which are the best indices for measuring species diversity?

28 October 2017 3,005 43 View

Does anyone know why I get different results for ANOSIM in Qiime and R?

Hello everyone, I have a 16S rRNA dataset of the gut microbiome of 11 humans, who were given two different dietary interventions. For beta diversity analysis I did ANOSIM of the Bray Curtis...

05 March 2017 8,800 2 View

Do you have some experiences with extraction of miRNAs from CSF?

11 July 2016 8,407 3 View

How do I combine data sets from miRNA target gene prediction tools, i.e. TargetScan, PicTar, Diana?

I'm trying to identify miRNA targets using online tools such as Targetscan, Pictar, Diana, miRDB. I am trying to find a final target list (intersect of all sub lists) in order to make...

12 June 2014 4,314 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View