I am looking for stains of anabaena that are documented to produce anatoxin-a. If anyone has a strain that I could acquire please message me. Thanks?

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How to explain the difference in LCIA results from ReCIPe2016 and IMPACT World+ with literature?

Hey there, I am currently conducting a life cycle assessment and as advised, I used different life cycle impact assessment (LCIA) methods to check for robustness of results. I found that when...

03 June 2024 8,431 2 View

Is there a download for DNA STRIDER 1.4?

Hi, Does anyone know where to find DNA STRIDER version 1.4? This is the old version that could align different sequences as well as search for oligos or shortersequences in a longer sequence. I...

30 April 2024 823 1 View

How do supply and demand curve for induced traffic look like?

There is various figures of supply and demand curves in literature that describe induced demand but they do not correspond to the properties of supply and demand of car travel. My question is...

02 May 2023 4,516 2 View

How to normalise and analyse the Fluo-4 calcium imaging data when cells were treated with agents 24 hours prior to imaging?

Hello, I am new to calcium imaging data analysis, and I need to analyse it for my master's project (I didn't actually acquire the calcium images myself). So in the experiments, the primary mouse...

08 June 2021 6,813 2 View

Can anyone suggest antibody against catalytic domain of human DNMT3A (amino acids P602-V912)?

Can anyone suggest antibody (suitable for WB, IF) against catalytic domain of human DNMT3A (amino acids P602-V912)? I went through several antibodies, but they were generated using synthetic...

03 December 2020 8,830 2 View

Simple 16S PCR-based fingerprinting method to identify bacterial diversity in stocks?

I am looking for a very simple approach to identify bacterial diversity in bacterial cultures. I have unselectively cultivated fecal samples based on a dilution series, so that only one single...

16 March 2020 1,207 4 View

When culturing magnetotactic bacteria, should I use D or L lactate?

Carbohydrate metabolism in bacteria, specifically magnetotactic bacteria. do they metabolize D or L forms preferentially?

04 November 2019 4,449 0 View

Can someone help to identify a representative sample size for a study that uses Fisher Exact?

Hello, can someone please help to identify a representative sample for my study, where I am using Fisher Exact to examine the significance of the association between two categorical variables? The...

18 March 2019 5,237 4 View

What software do you use to manage animal facility?

Hello, I am looking for tips for software to maintain some system in records from our animal facility. I am looking for something where I can put in information about mice (age, gender, strain,...

12 September 2018 1,426 1 View

Contamination of RNA after purifying it?

Hey, so it has happened a couple of times by now that I extracted RNA (RNA protect bacterial reagent, Qiagen) and DNase treated it with on column digestion using DNase I and a second DNase...

13 May 2018 8,945 3 View

Does exposure to toxins during pregnancy increase early puberty risk?

I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...

02 August 2024 8,361 0 View

What is the role of oxidative stress in toxin-induced carcinogenesis?

What is the role of oxidative stress in toxin-induced carcinogenesis? I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from...

02 August 2024 3,174 1 View

How do environmental toxins interact with genetic predispositions to increase cancer risk?

I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...

02 August 2024 2,627 1 View

Are current regulations effective in preventing cancer caused by toxins?

I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...

02 August 2024 4,474 2 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

Method of collection of samples of microorganisms from environmets to labortary?

can any one guid me about tractical method for preservation of microorganism same as bacteria, cyanobacteria and algea, in sampling from environment to Laboratory: 1- Sample collection 2-...

17 July 2024 3,687 11 View

Assistance with high taxonomic level identification of intertidal microalgae in SEM Images?

I am currently conducting research on benthic microalgae in the intertidal zone along the central California coast, specifically focusing on grazing by marine snails. As part of this study, I have...

08 July 2024 3,342 5 View

How can I improve purity of DNA extracted from cyanobacteria using Dynabeads?

I am trying to extract DNA from the cyanobacteria Microcystis aeruginosa using the Dynabeads DNA DIRECT Universal kit for the purpose of on-site detection. Yield is acceptable at ~100-500 ng/uL,...

01 July 2024 10,003 1 View

Which statistical test would be the best?

For my practical project at Uni, I am researching blue-green algae in a freshwater lake. I have three locations around the lake and am testing the water for phosphates, pH, and temperature. I am...

30 June 2024 4,264 3 View

Which MWCO filter to use to get rid of unwanted proteins?

I am working with snake venom, specifically targeting toxins around the 15 kDa range. I need to remove higher molecular weight toxins (above 20 kDa). Would it be advisable to use a 20 kDa MWCO...

05 June 2024 691 1 View