Carbohydrate metabolism in bacteria, specifically magnetotactic bacteria. do they metabolize D or L forms preferentially?
Hey there, I am currently conducting a life cycle assessment and as advised, I used different life cycle impact assessment (LCIA) methods to check for robustness of results. I found that when...
03 June 2024 8,431 2 View
Hi, Does anyone know where to find DNA STRIDER version 1.4? This is the old version that could align different sequences as well as search for oligos or shortersequences in a longer sequence. I...
30 April 2024 823 1 View
There is various figures of supply and demand curves in literature that describe induced demand but they do not correspond to the properties of supply and demand of car travel. My question is...
02 May 2023 4,516 2 View
Hello, I am new to calcium imaging data analysis, and I need to analyse it for my master's project (I didn't actually acquire the calcium images myself). So in the experiments, the primary mouse...
08 June 2021 6,813 2 View
Can anyone suggest antibody (suitable for WB, IF) against catalytic domain of human DNMT3A (amino acids P602-V912)? I went through several antibodies, but they were generated using synthetic...
03 December 2020 8,830 2 View
Learning to grow and identify toxin producing strains of cyanobacteria.
05 October 2020 2,160 2 View
I am looking for a very simple approach to identify bacterial diversity in bacterial cultures. I have unselectively cultivated fecal samples based on a dilution series, so that only one single...
16 March 2020 1,207 4 View
Hello, can someone please help to identify a representative sample for my study, where I am using Fisher Exact to examine the significance of the association between two categorical variables? The...
18 March 2019 5,237 4 View
Hello, I am looking for tips for software to maintain some system in records from our animal facility. I am looking for something where I can put in information about mice (age, gender, strain,...
12 September 2018 1,426 1 View
Hey, so it has happened a couple of times by now that I extracted RNA (RNA protect bacterial reagent, Qiagen) and DNase treated it with on column digestion using DNase I and a second DNase...
13 May 2018 8,945 3 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
30 July 2024 2,483 5 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
through genetic modification or metabolic engineering? Is it helpful, good for health ?
22 July 2024 7,811 4 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...
17 July 2024 3,785 9 View
I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer...
16 July 2024 5,031 1 View