Can anyone suggest antibody against catalytic domain of human DNMT3A (amino acids P602-V912)?

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How to explain the difference in LCIA results from ReCIPe2016 and IMPACT World+ with literature?

Hey there, I am currently conducting a life cycle assessment and as advised, I used different life cycle impact assessment (LCIA) methods to check for robustness of results. I found that when...

03 June 2024 8,431 2 View

Is there a download for DNA STRIDER 1.4?

Hi, Does anyone know where to find DNA STRIDER version 1.4? This is the old version that could align different sequences as well as search for oligos or shortersequences in a longer sequence. I...

30 April 2024 823 1 View

How do supply and demand curve for induced traffic look like?

There is various figures of supply and demand curves in literature that describe induced demand but they do not correspond to the properties of supply and demand of car travel. My question is...

02 May 2023 4,516 2 View

How to normalise and analyse the Fluo-4 calcium imaging data when cells were treated with agents 24 hours prior to imaging?

Hello, I am new to calcium imaging data analysis, and I need to analyse it for my master's project (I didn't actually acquire the calcium images myself). So in the experiments, the primary mouse...

08 June 2021 6,813 2 View

I am looking for stains of anabaena that are documented to produce anatoxin-a. If anyone has a strain that I could acquire please message me. Thanks?

Learning to grow and identify toxin producing strains of cyanobacteria.

05 October 2020 2,160 2 View

Simple 16S PCR-based fingerprinting method to identify bacterial diversity in stocks?

I am looking for a very simple approach to identify bacterial diversity in bacterial cultures. I have unselectively cultivated fecal samples based on a dilution series, so that only one single...

16 March 2020 1,207 4 View

When culturing magnetotactic bacteria, should I use D or L lactate?

Carbohydrate metabolism in bacteria, specifically magnetotactic bacteria. do they metabolize D or L forms preferentially?

04 November 2019 4,449 0 View

Can someone help to identify a representative sample size for a study that uses Fisher Exact?

Hello, can someone please help to identify a representative sample for my study, where I am using Fisher Exact to examine the significance of the association between two categorical variables? The...

18 March 2019 5,237 4 View

What software do you use to manage animal facility?

Hello, I am looking for tips for software to maintain some system in records from our animal facility. I am looking for something where I can put in information about mice (age, gender, strain,...

12 September 2018 1,426 1 View

Contamination of RNA after purifying it?

Hey, so it has happened a couple of times by now that I extracted RNA (RNA protect bacterial reagent, Qiagen) and DNase treated it with on column digestion using DNase I and a second DNase...

13 May 2018 8,945 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View