How to retrieve the associated diseases for a list of reference SNPs?

I have a list of reference SNPs IDs (rsid) and I need to retrieve the associated diseases ... what are the suitable bioinformatics tools or databases?

01 February 2020 4,835 1 View

How to get a Phased VCF file?

Can I phase VCF file after it has been generated ? Or I must have a phased BAM to get a phased VCF? Which tool is best used for this purpose?

09 October 2018 668 0 View

Gene list - disease association ?

I have a gene list and want to map it to human diseases to know what are the most probable disease relates to my list. What is the best bioinformatics tool to do this job?

05 June 2018 3,581 1 View

Is there a complete list of files used in the field of "omics" ?

I find different types of files in field of bioinformatics ... where can I find a complete list and description for these files?

02 March 2018 1,119 0 View

23 &Me variant names and genotypes ?

In 23&Me files for personal genetics services, I found some genetic variants named like (i6033918) and the genotype is (II). What does this mean?

09 October 2017 5,625 3 View

Where to find a database linking genetics and facial topology?

?

31 December 2016 9,682 1 View

Where to find a list of activators and inhibitors of an enzyme ?

I need a downloadable list of structures of activators and inhibitors of a certain enzyme ... where can I find a database containing this data ?

08 September 2016 325 4 View

How to detect the best variant affecting patient's response to a drug?

I detected a set of patients that have a high response to a drug. Each patient has 40 different reads ( 20 nominal and 20 continuous) resemble certain genetic and expression variations. How should...

03 April 2016 422 3 View

Some bioinformatics tools need Linux platform, others need Windows. What can I do for this?

Does anyone have an experience in getting Windows and Linux on the same computer (Laptop)?

10 November 2014 8,696 5 View

Is there a source where I can find the 3D structures of the different protein alleles?

I need 3d structures of different alleles of the same protein.

10 November 2014 1,115 3 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View