Can someone please look at it and tell me if it would be worthwhile pursuing this sample for hybridization for microarray?

Is it prudent to use an RNA sample which has no RIN number, when analyzed using a Bio-analyzer (Agilent). The profile shows a good 18S peak. However, the 28S peak is very small.(probably the...

06 July 2015 9,881 8 View

Can we anesthetize mice before giving oral gavage?

I am working with C57BL6 mice, which are little aggressive. I need to feed them by oral gavage. We have tried doing it in wake animals but they are giving us a tough time holding them and even if...

04 May 2015 8,720 11 View

Is anyone familiar with rosuvastatin in cell culture applications?

What is the ideal solvent and concentration in which rosuvastatin-calcium can be dissolved, for cell-culture applications? Is it soluble in water or do we need ethanol or DMSO solutions? If so...

09 October 2014 5,360 3 View

Can anyone help with Drabkin's assay standard hemoglobin solution and in vivo matrigel plug assay?

Can human hemoglobin be used to prepare the standard curve for Drabkin's assay to estimate the hemoglobin concentration in the matrigel plug injected in mice?

01 February 2014 4,940 0 View

Can someone advise on a storage of isolated PBMCs for microarray or NXG sequencing?

I want to store the isolated PBMCs of my study subjects in the best possible way that their RNA is preserved and in pristine state for microarray/NXG sequencing analysis. I tried snap freezing the...

31 December 2013 4,871 2 View

Redissolution of BSA after salting out with ammonium chloride?

Is it a common phenomenon that BSA redissolves after being salted out by ammonium sulphate upon increasing the concentration further? If so what could be the reason for it?

08 September 2013 1,314 0 View

Simultaneous analysis of cell surface marker expression and cell cycle analysis in PBMCs?

How to analyse freshly isolated peripheral blood mono nuclear cells simultaneously for CD34 expression and the cell cycle phase they are in. The options I have is to use propidium iodide or...

08 September 2013 6,462 2 View

Can someone suggest a protocol for proper detection of the nitrites using Griess assay?

I am trying to measure nitrite content in serum samples by Griess assay. But I am facing problems like curdling of serum (possibly due to plasma contamination). Do I have to do some pre-treatment...

07 August 2013 2,074 1 View

What are the markers for which staining has to be done to enumerate the rodent haematopoietic stem cells from peripheral blood?

Rat / mouse haematopoietic stem cell enumeration from peripheral blood (retro orbital bleeding) by flow cytometry. Please suggest any protocol or kits if available. Thank you.

07 August 2013 7,383 1 View

Hind limb ischemia in SCID mice?

We are facing a problem in induction of hind limb ischemia in SCID mice. We tried common femoral artery ligation (only knot) and also cut, but it is not inducing ischemia as expected. The limb is...

05 June 2013 6,158 15 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

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28 February 2021 9,936 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View