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Questions related from Mallu Abhiram Charan Tej
I am interested in finding a database where I can input a list of genes or gene IDs and get the information about how many and which of these genes are largely expressed in a particular cell type....
10 October 2018 610 3 View
Is it prudent to use an RNA sample which has no RIN number, when analyzed using a Bio-analyzer (Agilent). The profile shows a good 18S peak. However, the 28S peak is very small.(probably the...
07 July 2015 9,952 8 View
I am working with C57BL6 mice, which are little aggressive. I need to feed them by oral gavage. We have tried doing it in wake animals but they are giving us a tough time holding them and even if...
05 May 2015 8,794 11 View
What is the ideal solvent and concentration in which rosuvastatin-calcium can be dissolved, for cell-culture applications? Is it soluble in water or do we need ethanol or DMSO solutions? If so...
10 October 2014 5,433 3 View
Can human hemoglobin be used to prepare the standard curve for Drabkin's assay to estimate the hemoglobin concentration in the matrigel plug injected in mice?
02 February 2014 5,007 0 View
I want to store the isolated PBMCs of my study subjects in the best possible way that their RNA is preserved and in pristine state for microarray/NXG sequencing analysis. I tried snap freezing the...
01 January 2014 4,939 2 View
Is it a common phenomenon that BSA redissolves after being salted out by ammonium sulphate upon increasing the concentration further? If so what could be the reason for it?
09 September 2013 1,435 0 View
How to analyse freshly isolated peripheral blood mono nuclear cells simultaneously for CD34 expression and the cell cycle phase they are in. The options I have is to use propidium iodide or...
09 September 2013 6,526 2 View
I am trying to measure nitrite content in serum samples by Griess assay. But I am facing problems like curdling of serum (possibly due to plasma contamination). Do I have to do some pre-treatment...
08 August 2013 2,144 1 View
Rat / mouse haematopoietic stem cell enumeration from peripheral blood (retro orbital bleeding) by flow cytometry. Please suggest any protocol or kits if available. Thank you.
08 August 2013 7,459 1 View
We are facing a problem in induction of hind limb ischemia in SCID mice. We tried common femoral artery ligation (only knot) and also cut, but it is not inducing ischemia as expected. The limb is...
06 June 2013 6,224 15 View
I am interested in measuring insulin and nor-epinephrine levels in rodents (rat / mouse). What are the best methods or kits available, which use the least possible amounts of serum /plasma/blood...
06 June 2013 9,942 2 View
I would like to have a software to manage large amounts of clinical data.
05 May 2013 797 3 View
How many cells do I inject and if in PBS or any thing else. which route of injection is preferred?
04 April 2013 4,563 2 View
Which is a better option to run SDS PAGE gel...constant current or constant voltage. Please mention the values and give a reference if possible.
04 April 2013 4,565 24 View
I am interested in doing retro orbital bleeding of mice/rats for doing FACS analysis of the whole blood. I am looking at a rare population. Right now I am using chloroform to do the same but I am...
01 January 2013 3,702 31 View
We are having a problem in detecting the basal eNOS levels in freshly isolated PBMCs by RT-PCR or western blotting. Does anyone have suggestions for the same and a maybe a protocol?
01 January 2013 292 2 View
