Can someone suggest a protocol for proper detection of the nitrites using Griess assay?

Mallu Abhiram Charan Tej @Mallu_Abhiram_Charan_Tej

08 August 2013 1 2K Report

I am trying to measure nitrite content in serum samples by Griess assay. But I am facing problems like curdling of serum (possibly due to plasma contamination). Do I have to do some pre-treatment of the serum samples before actually doing the assay? I add serum sample and griess reagent in equal volumes and incubate them in dark for 10 mins at room temperature and take absorbance values at 548nm. please suggest any modifications in this procedure that are required.

Sharad Purohit

You can centrifuge the samples after reaction and read the absorbance. Alternatively, remove proteins by precipitation before doing griess reaction

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