09 September 2013 0 1K Report

Is it a common phenomenon that BSA redissolves after being salted out by ammonium sulphate upon increasing the concentration further? If so what could be the reason for it?

More Mallu Abhiram Charan Tej's questions See All
Is there a database that can give out cell specific gene expression from an input gene list?

I am interested in finding a database where I can input a list of genes or gene IDs and get the information about how many and which of these genes are largely expressed in a particular cell type....

09 October 2018 656 3 View

Can someone please look at it and tell me if it would be worthwhile pursuing this sample for hybridization for microarray?

Is it prudent to use an RNA sample which has no RIN number, when analyzed using a Bio-analyzer (Agilent). The profile shows a good 18S peak. However, the 28S peak is very small.(probably the...

06 July 2015 9,989 8 View

Can we anesthetize mice before giving oral gavage?

I am working with C57BL6 mice, which are little aggressive. I need to feed them by oral gavage. We have tried doing it in wake animals but they are giving us a tough time holding them and even if...

04 May 2015 8,843 11 View

Is anyone familiar with rosuvastatin in cell culture applications?

What is the ideal solvent and concentration in which rosuvastatin-calcium can be dissolved, for cell-culture applications? Is it soluble in water or do we need ethanol or DMSO solutions? If so...

09 October 2014 5,471 3 View

Can anyone help with Drabkin's assay standard hemoglobin solution and in vivo matrigel plug assay?

Can human hemoglobin be used to prepare the standard curve for Drabkin's assay to estimate the hemoglobin concentration in the matrigel plug injected in mice?

01 February 2014 5,043 0 View

Can someone advise on a storage of isolated PBMCs for microarray or NXG sequencing?

I want to store the isolated PBMCs of my study subjects in the best possible way that their RNA is preserved and in pristine state for microarray/NXG sequencing analysis. I tried snap freezing the...

31 December 2013 4,985 2 View

Simultaneous analysis of cell surface marker expression and cell cycle analysis in PBMCs?

How to analyse freshly isolated peripheral blood mono nuclear cells simultaneously for CD34 expression and the cell cycle phase they are in. The options I have is to use propidium iodide or...

08 September 2013 6,565 2 View

Can someone suggest a protocol for proper detection of the nitrites using Griess assay?

I am trying to measure nitrite content in serum samples by Griess assay. But I am facing problems like curdling of serum (possibly due to plasma contamination). Do I have to do some pre-treatment...

07 August 2013 2,194 1 View

What are the markers for which staining has to be done to enumerate the rodent haematopoietic stem cells from peripheral blood?

Rat / mouse haematopoietic stem cell enumeration from peripheral blood (retro orbital bleeding) by flow cytometry. Please suggest any protocol or kits if available. Thank you.

07 August 2013 7,503 1 View

Hind limb ischemia in SCID mice?

We are facing a problem in induction of hind limb ischemia in SCID mice. We tried common femoral artery ligation (only knot) and also cut, but it is not inducing ischemia as expected. The limb is...

05 June 2013 6,278 15 View

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