Can human hemoglobin be used to prepare the standard curve for Drabkin's assay to estimate the hemoglobin concentration in the matrigel plug injected in mice?
I am interested in finding a database where I can input a list of genes or gene IDs and get the information about how many and which of these genes are largely expressed in a particular cell type....
09 October 2018 656 3 View
Is it prudent to use an RNA sample which has no RIN number, when analyzed using a Bio-analyzer (Agilent). The profile shows a good 18S peak. However, the 28S peak is very small.(probably the...
06 July 2015 9,989 8 View
I am working with C57BL6 mice, which are little aggressive. I need to feed them by oral gavage. We have tried doing it in wake animals but they are giving us a tough time holding them and even if...
04 May 2015 8,843 11 View
What is the ideal solvent and concentration in which rosuvastatin-calcium can be dissolved, for cell-culture applications? Is it soluble in water or do we need ethanol or DMSO solutions? If so...
09 October 2014 5,471 3 View
I want to store the isolated PBMCs of my study subjects in the best possible way that their RNA is preserved and in pristine state for microarray/NXG sequencing analysis. I tried snap freezing the...
31 December 2013 4,985 2 View
Is it a common phenomenon that BSA redissolves after being salted out by ammonium sulphate upon increasing the concentration further? If so what could be the reason for it?
08 September 2013 1,490 0 View
How to analyse freshly isolated peripheral blood mono nuclear cells simultaneously for CD34 expression and the cell cycle phase they are in. The options I have is to use propidium iodide or...
08 September 2013 6,565 2 View
I am trying to measure nitrite content in serum samples by Griess assay. But I am facing problems like curdling of serum (possibly due to plasma contamination). Do I have to do some pre-treatment...
07 August 2013 2,194 1 View
Rat / mouse haematopoietic stem cell enumeration from peripheral blood (retro orbital bleeding) by flow cytometry. Please suggest any protocol or kits if available. Thank you.
07 August 2013 7,503 1 View
We are facing a problem in induction of hind limb ischemia in SCID mice. We tried common femoral artery ligation (only knot) and also cut, but it is not inducing ischemia as expected. The limb is...
05 June 2013 6,278 15 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
05 August 2024 454 3 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...
04 August 2024 4,130 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View