Hi, is there anyone who wants assistance with interpretation and/or classification of mutations to determine possible causality?

While doing research on genes possibly involved in a specific phenotype, you can run in to several difficulties for the interpretation and classification of mutations found, consequently making...

09 October 2017 9,968 2 View

Is there a simple way to determine if a mutation introducing a premature stopcodon signals nRNA degragdation?

11 December 2014 967 6 View

Would it be possuible to determine why two homologous proteins show a high degree of species specificity for the same ligand?

If two proteins are known for there distinct functions, but show affinity for the ligand of at least one other between species (bovine>human), could this be analysed?

04 May 2014 2,061 5 View

What is or are the best bioinformatic tools to assess the structural and functional implication of a non-synonymous mutations in humans?

There are several tools available to predict such implication, but the why of using one or even more remains unclear to me. Does someone have an idea of "the best" methods to be used. Moreover, is...

03 April 2014 7,441 10 View

Protein-protein interactions: are there any reliable programs to predict interaction between proteins using homology-based molecular structures?

I want to know how two proteins of interst interact with each other by using their homology-based molecular structure. I know from experimental data which part of each protein is required for...

03 April 2014 2,878 11 View

Does anyone have some general info on non-coding RNAs?

I would like to get some key articles and/or presentations to help understand the underlying mechanism responsible that is responsible for transcription and their function.

31 December 2013 8,669 2 View

Looking for protein-protein docking software. Any suggestions and experiences?

Would like to get some assistance with simulating/estimating the interactions between two proteins to beteer understand structure-function properties.

09 October 2013 296 8 View

Where to publish your data?

If you have data on phylogenetics, functional divergence and 3d structures, where should you publish it?

09 October 2013 4,381 7 View

How to write a good grants proposal?

I want to continue my career in Academia in the field of bioinformatics.

09 October 2013 8,134 4 View

I want to be a reviewer for journals in bioinformatics and computational biology. Any options?

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09 October 2013 9,470 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View