What's the longest insert you put into a plasmid via quickchange-like methods?

We would like to put a 12-20 aminoacid linker into a recombinant protein expressed in E coli. We've had good experience with Quickchange-like protocols; but never tried with 36-60 nucleotide...

02 March 2019 3,185 4 View

Is Lithium Lauryl Sulfate OK to run K+-containing samples on SDS-PAGE?

Hi all, Can one use Li Lauril Sulfate (LDS) in loading buffer to run protein samples containing high concentrations of KCl, on SDS-PAGE? Does one need to replace SDS in the running buffer and in...

01 February 2019 1,614 6 View

Can you recommend a tabletop front-loading autoclave?

Can anyone recommend a front-loading tabletop autoclave which could be used to sterilize up to six 2L flasks at once with steam? Looking for something that is robust, simple, and ages well (i.e....

07 August 2018 6,653 0 View

Suggest a method for harvesting of adherent cells from hollow fiber modules? Does scraping work? Would using hydrophobic fibers help?

We are looking for a cost-effective way to grow adherent cells (HCT116, US2OS), in amounts of 1*10^10, for subsequent isolation of nuclei and preparation nuclear extracts per Dignam 1980....

05 June 2018 7,168 5 View

Has anyone tried using standard hollow fiber PES hemo-dialysis cartridges to culture adherent mammalian cells?

The surface area (1-2 sq meters) and the basic specs (excluded volume, PES memberane chemistry, pressure drop, non-fowling, sterile, QC, Mw rejection) of hollow fiber dialysis filters appears...

05 June 2018 9,437 0 View

Can 5+M guanidine-HCl denature dsDNA PCR products upon prolonged exposure (>16 hours, room temp, this is for crush and soak of acrlamide gels)?

I am wondering if prolonged incubations in 5M GuHCl when crush-and soaking acrylamide gel slabs can denature DNA. I normally use water or the Maniatis buffer, but having 5M GuHCl around would help...

02 March 2018 7,428 3 View

Does anyone Happen to know what BioRad's iProof PCR enzyme is (beyond what's in the catalog description)?

09 October 2016 1,608 2 View

Does anyone have experience with the mini8 PCR cycler?

09 October 2016 3,488 0 View

How to control pH and composition of PEG solutions?

Hi all, I am trying to concentrate a DNA sample using dialysis against 50% PEG, through a 10K cut-off membrane. I made a 50% solution of PEG20K supplemented with 10mM Tris (target pH=7.8), and...

08 September 2016 3,873 10 View

An affordable and reliable thermal cycler?

02 March 2016 3,750 5 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

No title

02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View