03 March 2016 5 4K Report

I've been wondering whether there are any new manufacturers around who make reliable, affordable PCR machines. The Chinese now make great smartphones, may be they now make good cyclers, too (that cost way under $5,000...) Any suggestions?

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Can you recommend a tabletop front-loading autoclave?

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Is there a chemically defined, FBS-free media formulation for HCT-116 and U2OS cells (adherent human cells)?

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Suggest a method for harvesting of adherent cells from hollow fiber modules? Does scraping work? Would using hydrophobic fibers help?

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05 June 2018 7,305 5 View

Has anyone tried using standard hollow fiber PES hemo-dialysis cartridges to culture adherent mammalian cells?

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Can 5+M guanidine-HCl denature dsDNA PCR products upon prolonged exposure (>16 hours, room temp, this is for crush and soak of acrlamide gels)?

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Does anyone Happen to know what BioRad's iProof PCR enzyme is (beyond what's in the catalog description)?

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Does anyone have experience with the mini8 PCR cycler?

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How to control pH and composition of PEG solutions?

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