The surface area (1-2 sq meters) and the basic specs (excluded volume, PES memberane chemistry, pressure drop, non-fowling, sterile, QC, Mw rejection) of hollow fiber dialysis filters appears pretty similar to 'preparative scale' hollow fiber cell culture modules. Dyalysis cartridges might be a cost effective alternative, since they are mass-produced and, I believe, were the original devices in which hollow fiber culture was first tried in the 1970s in the first place. Thus, I was wondering how wacky of an idea it would be to try culturing adherent mammalian cells in standard (e.g. Fresenius) PES modules. This is not for antibody production, I just need the cells to stick, expand 20x, and get taken out with trypsin/accutase.
- Badges
- Science topic
More Andrey Revyakin's questions See All
Similar questions and discussions