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Questions related from Andrey Revyakin
We would like to put a 12-20 aminoacid linker into a recombinant protein expressed in E coli. We've had good experience with Quickchange-like protocols; but never tried with 36-60 nucleotide...
03 March 2019 3,280 4 View
Hi all, Can one use Li Lauril Sulfate (LDS) in loading buffer to run protein samples containing high concentrations of KCl, on SDS-PAGE? Does one need to replace SDS in the running buffer and in...
02 February 2019 1,761 6 View
Can anyone recommend a front-loading tabletop autoclave which could be used to sterilize up to six 2L flasks at once with steam? Looking for something that is robust, simple, and ages well (i.e....
08 August 2018 6,747 0 View
FBS-free medium is what I am looking for. If defined growth factors are required, I can express them (if they are available in recombinant form). Artificial meat companies seem to have figured it...
07 July 2018 7,196 4 View
We are looking for a cost-effective way to grow adherent cells (HCT116, US2OS), in amounts of 1*10^10, for subsequent isolation of nuclei and preparation nuclear extracts per Dignam 1980....
06 June 2018 7,254 5 View
The surface area (1-2 sq meters) and the basic specs (excluded volume, PES memberane chemistry, pressure drop, non-fowling, sterile, QC, Mw rejection) of hollow fiber dialysis filters appears...
06 June 2018 9,532 0 View
I am wondering if prolonged incubations in 5M GuHCl when crush-and soaking acrylamide gel slabs can denature DNA. I normally use water or the Maniatis buffer, but having 5M GuHCl around would help...
03 March 2018 7,511 3 View
For example, is it identical to NEB's phusion? The latter is described in: http://barricklab.org/twiki/bin/view/Lab/ProtocolsReagentsPhusion
10 October 2016 1,703 2 View
I am looking to buy a simple PCR cycler. Was considering OpenPCR, but their web site seems to not have been updated for a while, and they do not return emails. Does anyone have experience with...
10 October 2016 3,578 0 View
Hi all, I am trying to concentrate a DNA sample using dialysis against 50% PEG, through a 10K cut-off membrane. I made a 50% solution of PEG20K supplemented with 10mM Tris (target pH=7.8), and...
09 September 2016 3,956 10 View
I've been wondering whether there are any new manufacturers around who make reliable, affordable PCR machines. The Chinese now make great smartphones, may be they now make good cyclers, too...
03 March 2016 3,880 5 View
