Hi everyone,
I'm trying to figure out if there is a cheapier version of a cell freezer (that drops 1°C each minutes). We have the Nalgene version with isopropanol and also some Corning (in styrofoam), but my lab need to buy a lot more for a ongoing experiment. I can't believe the price of those things, especially the styrofoam version. Do you know any other alternatives (different brands) that offer the same conditions to the cells?
Let me know if do!
Thanks a lot!
Marc-Alexandre Lafrance
Master student
Infection disease research center
Quebec, Canada
You can look at this researchgate trend:
https://www.researchgate.net/post/How-can-I-cryopreserve-HepG2-cells-without-liquid-nitrogen-or-mr-frosty
Sigma-Aldrich also seems to recommend putting the cells in an insulated box at -80C.
They also seem to recommend a method with Nitrogen vapor.
Here is the link:
https://www.sigmaaldrich.com/technical-documents/protocols/biology/cryopreservation-of-cell-lines.html?gclid=CjwKCAjw7J6EBhBDEiwA5UUM2ta-OJMP5QHti5pDkNs6GZIDLqpSOS3I6IJgcwscgy4VS2xfMIhbiBoCfMUQAvD_BwE
I have also heard about putting isopropanol in a styrofoam box, though I am not fully aware how that works... It just seems like the ghetto version of the Mr. Frosty.
If these options do not seem good to you, you can also borrow Mr. Frosties from neighbouring laboratories of your research institution.
Hope this helps,
Rose
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