Or any marker at all? It seems like all the usual suspects, Cd11b, Iba1, PU.1, increase with inflammation. Would CX3CR1 be an inflammation-independent microglial marker?
try isolectin B4 from sigma, even that the receptor is unknown, clearly do not change with activation
Thanks! Is there a publication for that use? I've used tomato lectin in the past, but I believe that that increases with microglial activation.
that's a hard question. Why don't you try a channel or neurotransmitter receptor ? All molecules that are commonly known usually change...
CX3CR1 also changes, as do the purinergic receptors. I agree that isolectin may be the best chance.
From what I've read, the combination of CX3CR1+, CD45(lo), CCR2(absent), and Cd11b+ may work for us. I'll also try the isolectin. Thanks!
ah...you're doing flow, sorry I didn't catch that. Yes, the combination you mentioned is good. I've never used the lectin for facs.-only for immuno Good luck!
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