I recently reactivated our AKTA Pure (after we moved it to a new bench), but the AKTA is suddenly not recognizing the F9-C fraction collector. (It was working well before.) I can hear the fraction collector arm trying to move, but it does not. The AKTA shows the error message "(Error) Hardware Manager: Fraction Collector Arm (F9-A) : (30) The fractionation arm failed to find the home position."
I checked the tubing from the fraction collector to the AKTA and the cable from the fraction collector to the AKTA, and they are connected. I checked the system properties, and the enabled components of the system look correct. I manually purged all the valves with ethanol. I restarted the AKTA and the computer several times, but I get the same message. Has anyone experienced this, and how did you fix it? Thanks.
To connect the AKTA Pure chromatography system to the F9-C fraction collector, follow these steps:
1. Turn off the AKTA Pure system and disconnect it from the power source.
2. Locate the fraction collector port on the back of the AKTA Pure system. This should be a 25-pin D-sub connector labeled "Fraction Collector".
3. Connect one end of a serial cable to the fraction collector port on the AKTA Pure system.
4. Connect the other end of the serial cable to the serial port on the F9-C fraction collector.
5. Turn on the AKTA Pure system and wait for it to complete the startup procedure.
6. Configure the AKTA Pure system to communicate with the F9-C fraction collector. This can be done using the AKTA Pure system control software.
7. In the software, select the "Fraction Collector" option from the list of devices and configure the communication settings, such as the baud rate and parity.
8. Once the communication settings are configured, the AKTA Pure system should be able to communicate with the F9-C fraction collector and control the collection of fractions during the chromatography run.
It is important to ensure that the serial cable used to connect the AKTA Pure system to the F9-C fraction collector is of the correct type and length, as specified by the manufacturer. Additionally, it is important to follow all safety precautions and instructions during the setup process to avoid damage to the equipment or injury to personnel.
1. Turn off both the AKTA Pure and the F9-C fraction collector.
2. Locate the communication cable that came with the F9-C fraction collector. The cable will have a DB-9 connector on one end and an RJ-45 connector on the other end.
3. Connect the DB-9 connector of the communication cable to the RS-232 port on the back of the F9-C fraction collector.
4. Connect the RJ-45 connector of the communication cable to the RS-232 port on the back of the AKTA Pure system.
5. Turn on the F9-C fraction collector, and then turn on the AKTA Pure system.
6. Once both systems are powered on, navigate to the "System Configuration" menu on the AKTA Pure system.
7. Select "Hardware Configuration" and then choose "F9-C Fraction Collector" from the drop-down menu.
8. Enter the appropriate communication settings for the F9-C fraction collector, such as the baud rate, data bits, stop bits, and parity. These settings can usually be found in the F9-C fraction collector user manual.
9. Save the changes, and then exit the menu.
10. You should now be able to control the F9-C fraction collector from the AKTA Pure system. To test the connection, try running a sample purification and verify that the fractions are being collected correctly by the F9-C fraction collector.
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Physical Phenomena
- Luminescence
- Fluorescence