RNA-Seq for the analysis of ribosomal RNA (rRNA) processing sites?

Greetings! I want to study ribosomal RNA expression and potential processing sites in bacteria. To such effect, I want to perform an RNA-Seq experiment in order to analyze potential rRNA...

02 December 2020 1,310 2 View

How to quantify percentage of DAB positive cells in IHC? Is there any automated tool?

Hi! I'm analyzing some TMA IHC cores using an scoring method that combines intensity and proportion of stained cells. I would like to know if is there any protocol to quantify percentage of DAB...

25 April 2020 479 2 View

T-test or one way ANOVA in RT-qPCR gene expression?

Hello, I checked the expression of several genes from different strains and I obtained the values of the relative normalized expression of each gene from each strain compared to the relative gene...

28 April 2019 3,598 3 View

How do i set up Biological replicates using CFX Maestro Software Bio-Rad?

Hello everybody, I am performing gene expression analysis using the Bio-Rad CFX maestro Software. I have different strains which I set up as "biological groups". In different plates I put my...

13 January 2019 5,685 2 View

How much sample should be used in a no RT control?

I would like to know when running a no rt control for qPCR should the amount of template used be the same as the amount used to make cDNA or the amount of cDNA used in the qPCR. I use 100ng of RNA...

15 October 2018 7,022 3 View

What is the toxicity of Sambucus nigra leaves, shoots and roots? Is the amount of cyanogenic glycosides in a given speciman variable?

Need a clear answer. I am seeing conflicting information on this.

19 October 2016 7,275 7 View

Replacing a standard curve reagent in validated assay?

17 October 2016 5,744 6 View

Copan eNAT swabs for microbiome work?

Hi all, So I've been working up a lab protocol for extracting DNA from oral and nasal swabs for 16S sequencing. Just when I thought I'd made my decision on swabs to use etc I get a recommendation...

26 September 2016 4,651 2 View

Optimising a competitive ELISA for sensitivity?

20 June 2016 8,127 10 View

Does the presence of trypsin degrade cytokines?

I have samples of frozen tissue supernatant that are being analyzed for cytokine production. However, the cells have been treated with trypsin. Will this affect the concentration of cytokines once...

15 June 2016 2,381 6 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View

How to find natural frequency of a bridge from vehicle induced vibrations?

i have frequencies of bridge that are effected by mass of vehicle. the problem is when any vehicle pass the bridge it would add the its mass to the bridge mass ultimately mass of the bridge will...

22 February 2021 10,004 3 View

Did any urologists have experienced with retroperitonel leiomyosarcoma from renal vein with IVC enxtension?

Recently, we got one lady with right side retroperitoneal mass involving renal vein and extends into IVC upto diaphragm. CT suggested leiomyosarcoma. Right kidney separately seen from mass and it...

19 February 2021 6,873 1 View

How can I import .sav file into LISREL?

I've just downloaded free trial version of LISREL 10.3 but I got stuck with importing .sav (IBM SPSS 25.) file. I mainly followed the steps of this video...

15 February 2021 752 2 View

How do you interpret theoretical vs. experimental reaction orders with respect to a reactant?

I recently came across a paper focusing on the hydrodeoxygenation of oxygen-containing compounds. The paper linearized the rate equation in order to determine the experimental reaction order with...

14 February 2021 8,254 2 View

Can i use the " weight moisture" ( humidite ponderale in French) of plants to describe their efficiency in using water?

Weight moisture is : water content/ dry weight , if for example it is 4 it means that this plant have for each 1gam of dry mass 4 grams of water. can i use this to interpret the plant use of...

14 February 2021 5,784 1 View