T-test or one way ANOVA in RT-qPCR gene expression?

Hello, I checked the expression of several genes from different strains and I obtained the values of the relative normalized expression of each gene from each strain compared to the relative gene...

28 April 2019 3,598 3 View

How do i set up Biological replicates using CFX Maestro Software Bio-Rad?

Hello everybody, I am performing gene expression analysis using the Bio-Rad CFX maestro Software. I have different strains which I set up as "biological groups". In different plates I put my...

13 January 2019 5,685 2 View

How much sample should be used in a no RT control?

I would like to know when running a no rt control for qPCR should the amount of template used be the same as the amount used to make cDNA or the amount of cDNA used in the qPCR. I use 100ng of RNA...

15 October 2018 7,022 3 View

What is the toxicity of Sambucus nigra leaves, shoots and roots? Is the amount of cyanogenic glycosides in a given speciman variable?

Need a clear answer. I am seeing conflicting information on this.

19 October 2016 7,275 7 View

Replacing a standard curve reagent in validated assay?

17 October 2016 5,744 6 View

Copan eNAT swabs for microbiome work?

Hi all, So I've been working up a lab protocol for extracting DNA from oral and nasal swabs for 16S sequencing. Just when I thought I'd made my decision on swabs to use etc I get a recommendation...

26 September 2016 4,651 2 View

Optimising a competitive ELISA for sensitivity?

20 June 2016 8,127 10 View

Does the presence of trypsin degrade cytokines?

I have samples of frozen tissue supernatant that are being analyzed for cytokine production. However, the cells have been treated with trypsin. Will this affect the concentration of cytokines once...

15 June 2016 2,381 6 View

How do I solve my problem with my ChIPs?

01 September 2015 287 5 View

Why do I have flaky DNA clumps upon Ethanol precipitation?

I did a large scale (6 L) extraction of DNA using Phenol/CIA and PEG precipitations. The step before the ethanol precipitation is CIA extraction. After adding the 2.5X ethanol the DNA looks...

19 March 2015 9,510 2 View

Which tropical country is the most easy for obtaining research permit (for biological research)?

Dear all, High beurocracy hihly complicate field ecolocial and evolutionary research nowadays. Nearly all tropical countries (maybe all?) requires research permit for performitng research and in...

04 February 2021 3,672 6 View

How to distinguish pure and adulterated honey using NMR spectroscopy?

What are the common adulterants in commercial honey? how to identify pure honey and adulterated honey with the help of NMR spectroscopy? Is there any other technique to check purity of honey?

20 January 2021 5,456 12 View

Can R be used for SEO?

13 January 2021 1,920 1 View

How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for Metabarcoding?

I'm working on pollen DNA metabarcoding to identify the floral composition of honey. How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for the...

28 December 2020 9,132 1 View

Santolina species, wax moth and bees?

Expect a savage answer and being reported to ResearchGate Admin if your answer clearly shows you did not read the whole question and you are scamming the points system by answering with an...

12 December 2020 4,413 7 View

What was scientist thinking about Bees communication before Karl von Frisch discovery?

In the 1920s, Karl von Frisch pointed out that bees use special dancing patterns. What scientist or people think about bees communication before its discovery.

22 November 2020 1,426 5 View

LIFE in SMART CITIES: Animal data versus Human data, complementarity or not?

One observation is that in any city (those I know) you have humans, of course, but you also have many animals. Statistics of humans start with population data (for instance demographics) and...

08 November 2020 4,127 6 View

Can anyone help me understand how to adjust my SAS glm analysis to include a random effect for site?

I am not great with statistics so please be understanding. I have 17 pollinator sites that I collected data from and am comparing the effect of habitat type (2 classes) on several different...

24 September 2020 3,140 5 View

Flight direction/pattern of moth?

I would like to determine the movement of moths in response to mating disruption pheromone traps. Pheromone lures (matting disruption) were placed in the center of the treatment plot. High...

23 September 2020 6,293 4 View

Labs for purification, identification & isolation of unknown proteins form native sample of honey and to test versus GS-9L cells in vitro?

I asked for protein purification, identification and isolation of unknown proteins and test versus GS-9L other than that we already got in our patent WO/2014/040605 - form native sample of honey?!

23 September 2020 1,839 3 View