Hi everyone, I am currently using Genemapper V 6 to analysis microsatellite data. While we are interested in the allele sizes, we are also interested in the peak height so we can look at ratios between peaks for heterozygous individuals. Genemapper is fine, but I was wondering if there were other fragment analysis softwares out there that can also do this? I have looked into Geneious (it does not) and Osiris (can't seem to find out if if does). Any help would be appreciated!
Geneious does show the peaks' height, but I do not see a way to automatically view or export those values for each sample. Once you hover over a peak, it will show its height and the Y axis has a height scale. If you do not find any other software and if you do not have a crazy number of samples, you could try writing it down manually.
I'm not sure if the Generous tutorials have an extra info for:
https://www.geneious.com/features/microsatellite-genotyping/
My lab actually used GeneMarker for fragment analysis:
https://www.biogene.com/genemarker.html
- Badges
- Science topic