Review and cite DOSING protocol, troubleshooting and other methodology information | Contact experts in DOSING to get answers
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My protein is a transmembrane protein, therefore i need to construct a transmembrane using CHARMM-GUI. Can someone please suggest me a tutorial for the same.
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Is it possible to achieve this by combining flux tally and reaction rate cards?
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I am doing a DNA extraction from potato leaves infected with Phytophthora infestans for quantification via qPCR. The protocol includes two buffers: first, the tissue is disgragated with an...
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I am curious to know when will we have an AI Professor who will completely teach the students in the classroom instead of a Human Professor who is not perfect and makes mistakes.
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Hi I am trying to do fingerprinting of rhizobial isolates with the help of eric primer..I prepared a master mix..10x buffer-2.5ul dNTP(10mM) 2ul MgCl2(50mM) 1ul Taq(5U) 0.2 Template 3ul and Eric R...
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In the molecular dynamics simulation analysis part, how to calculate molecular surface area (MolSA) and polar surface area (PSA) in gromacs ?
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and Why do double bonds reduce it ?
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Hi to all I need a help in explaining how I can calculate the formation energy for a half Heusler compound using WIEN2k code
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Antiplatelet Therapy: Mechanisms, Indications, Administration What is the role of pharmacists in the management ofantiplatelet therapy, particularly in terms of drug selection,dosing, and...
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We produced scFv of an antibody from bacteria. Now we want to establish dissociation Kd of antibody using quantitative elisa. How can i know the expected Kd value (range in nM, or mM)?
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Is a person with a history nodular gastritis, which has been through nodular regression (according to endoscopy) because of sucessful H.pylori treatment, has the same prognosis as a person with no...
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We crossed SynCre mice with Tg403-loxp-rtTA mice, but all the pups ended up with either one of the genes, not both together, as confirmed by genotyping tests. Has this ever happened to anyone else?
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How to calculate dosing of polyherbal formulation and what quantity of individual herbs should be taken to prepare PHF.
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What are the introductory papers on water pollution?
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I found articles about intraperitoneal dose of an antioxidant. There is no information about otoprotective intratympanic dose of this chemical. How can I decide intratympanic dose of this...
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Lipofectamine is a commonly used transfection reagent known for its efficiency in delivering nucleic acids into mammalian cells. However, the optimal transfection conditions can vary depending on...
12 April 2024 5,810 2 View
I am performing some RT-qPCR with RNA transcripts (HIV-integrase) and as a control (RCAS transcripts). The amplification curve for my HIV int has low y-axis values. To rule out the possibility of...
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I have human, non-union tissues to be stained for sclerostin. The tissues were collected in 10% formalin and processed via paraffin embedding. Any recommendations for antibodies are much appreciated
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We are interested in detecting aggrecan (250 KDa) by WB. No luck so far, are there any recommendations, such as running buffer, sample loading buffer? Thank you in advance!
06 April 2024 6,181 4 View
I need assistance regarding respiration rates in kumquat at the recommended storage temperature i.e. 4-5 °C.
20 March 2024 9,972 0 View
Hello,Goal is finding concentration of PS20, prepared std curve concentrations of , 25,50,100,150 and 200 ug/mL , after methylene chloride extraction of PS20, and reading at UV spectromete at...
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calculation of switching And conduction losses in a three phase inverter. and also how to calculate these two losses in matlab. means how to prove theoretically and practically
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Can bacteria increase soil fertility and how microbes help in cleaning the environment and maintaining nitrogen balance in nature?
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