Review and cite DOSING protocol, troubleshooting and other methodology information | Contact experts in DOSING to get answers
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My protein is a transmembrane protein, therefore i need to construct a transmembrane using CHARMM-GUI. Can someone please suggest me a tutorial for the same.
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Is it possible to achieve this by combining flux tally and reaction rate cards?
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I am doing a DNA extraction from potato leaves infected with Phytophthora infestans for quantification via qPCR. The protocol includes two buffers: first, the tissue is disgragated with an...
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I am curious to know when will we have an AI Professor who will completely teach the students in the classroom instead of a Human Professor who is not perfect and makes mistakes.
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Hi I am trying to do fingerprinting of rhizobial isolates with the help of eric primer..I prepared a master mix..10x buffer-2.5ul dNTP(10mM) 2ul MgCl2(50mM) 1ul Taq(5U) 0.2 Template 3ul and Eric R...
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In the molecular dynamics simulation analysis part, how to calculate molecular surface area (MolSA) and polar surface area (PSA) in gromacs ?
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and Why do double bonds reduce it ?
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Hi to all I need a help in explaining how I can calculate the formation energy for a half Heusler compound using WIEN2k code
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Antiplatelet Therapy: Mechanisms, Indications, Administration What is the role of pharmacists in the management ofantiplatelet therapy, particularly in terms of drug selection,dosing, and...
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We produced scFv of an antibody from bacteria. Now we want to establish dissociation Kd of antibody using quantitative elisa. How can i know the expected Kd value (range in nM, or mM)?
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Is a person with a history nodular gastritis, which has been through nodular regression (according to endoscopy) because of sucessful H.pylori treatment, has the same prognosis as a person with no...
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We crossed SynCre mice with Tg403-loxp-rtTA mice, but all the pups ended up with either one of the genes, not both together, as confirmed by genotyping tests. Has this ever happened to anyone else?
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How to calculate dosing of polyherbal formulation and what quantity of individual herbs should be taken to prepare PHF.
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What are the introductory papers on water pollution?
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I found articles about intraperitoneal dose of an antioxidant. There is no information about otoprotective intratympanic dose of this chemical. How can I decide intratympanic dose of this...
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Lipofectamine is a commonly used transfection reagent known for its efficiency in delivering nucleic acids into mammalian cells. However, the optimal transfection conditions can vary depending on...
12 April 2024 5,811 2 View
I am performing some RT-qPCR with RNA transcripts (HIV-integrase) and as a control (RCAS transcripts). The amplification curve for my HIV int has low y-axis values. To rule out the possibility of...
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I have human, non-union tissues to be stained for sclerostin. The tissues were collected in 10% formalin and processed via paraffin embedding. Any recommendations for antibodies are much appreciated
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We are interested in detecting aggrecan (250 KDa) by WB. No luck so far, are there any recommendations, such as running buffer, sample loading buffer? Thank you in advance!
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I need assistance regarding respiration rates in kumquat at the recommended storage temperature i.e. 4-5 °C.
20 March 2024 9,976 0 View
Hello,Goal is finding concentration of PS20, prepared std curve concentrations of , 25,50,100,150 and 200 ug/mL , after methylene chloride extraction of PS20, and reading at UV spectromete at...
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calculation of switching And conduction losses in a three phase inverter. and also how to calculate these two losses in matlab. means how to prove theoretically and practically
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Can bacteria increase soil fertility and how microbes help in cleaning the environment and maintaining nitrogen balance in nature?
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