I'm looking for a cell line that would contain an integrated SIV, with a known number of SIV copies, other than 3D8. Much like ACH2, which contains 1 copy HIV, except SIV. Any suggestions?
Yes, I would assume. Just go to any reference source/data base (e.e. PubMed) type in your inquiry. With known number of copies - that is a tough one - you might have look at several lines if that information exist.
Also might be worth checking with NIH AIDS Reagent Program, e.g.
https://www.aidsreagent.org/reagentdetail.cfm?t=cell_lines&id=355
If the HIV or SIV is not defective it can replicate and thus change copy number of integrations. There are probably dozens of ways to create a cell line with one single integrated copy of a single viral genome, but any normal T-cell with a non-defective retrovirus insertion can change very rapidly.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line