I can't find it....
Aqua Live/Dead binds free amines. Will it bind free amines in the antibodies that are used in the same panel, so it's best to stain sequentially?
31 December 2017 1,226 1 View
Does anyone know of an instrument that would perform a fully automated isolation of PBMC's from whole blood?
04 May 2016 8,072 1 View
As I dilute my qPCR template, the plateau of the curves also goes down. Does this mean my primers are too low, primers form dimers, DNA quality is poor, all of the above?
04 May 2016 4,301 3 View
I'm looking for a cell line that would contain an integrated SIV, with a known number of SIV copies, other than 3D8. Much like ACH2, which contains 1 copy HIV, except SIV. Any suggestions?
04 May 2016 9,074 3 View
Trying to lyse a single cell INCLUDING NUCLEUS, reverse transcribe, and qPCR, all in the same reaction, with a purpose of detecting short cDNA and long gDNA fragments. Looks like lysing in the...
02 March 2016 623 4 View
Anyone knows of a HeLa cell line that expresses GFP under HIV-1 activated promoter? I'm trying to find a flow-based infectivity assay that would be comparable to HeLa TZM blue assay, with Gal or...
02 March 2016 2,630 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
