Should Aqua Live/Dead stain be separate from the other antibodies?

01 January 2018 1 1K Report

Aqua Live/Dead binds free amines. Will it bind free amines in the antibodies that are used in the same panel, so it's best to stain sequentially?

Matthew Stephens

Aqua live dead stains can be used in tandem with other antibody stains. If you have concerns you could also use Pacific blue or propidium iodide to sort one from the other. Hope it helps!

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