Should Aqua Live/Dead stain be separate from the other antibodies?

Aqua Live/Dead binds free amines. Will it bind free amines in the antibodies that are used in the same panel, so it's best to stain sequentially?

31 December 2017 1,154 1 View

Does anyone know the sequence of HIV-1 in U1 a.k.a. U937 cell line?

I can't find it....

31 December 2016 2,683 0 View

What is a likely cause of the variation in plateau of qPCR curves?

As I dilute my qPCR template, the plateau of the curves also goes down. Does this mean my primers are too low, primers form dimers, DNA quality is poor, all of the above?

04 May 2016 4,179 3 View

Is there a cell line with integrated SIV?

I'm looking for a cell line that would contain an integrated SIV, with a known number of SIV copies, other than 3D8. Much like ACH2, which contains 1 copy HIV, except SIV. Any suggestions?

04 May 2016 8,998 3 View

Conditions for Single cell lysis (including nucleus lysis)-RT-qPCR?

Trying to lyse a single cell INCLUDING NUCLEUS, reverse transcribe, and qPCR, all in the same reaction, with a purpose of detecting short cDNA and long gDNA fragments. Looks like lysing in the...

02 March 2016 546 4 View

Is there a HeLa line with GFP induced by HIV-1?

02 March 2016 2,564 2 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View