Hi, everyone.
first, i'm so sorry about i can't use English well...:(
I research cancer cells and i culture breast cancel cell Sk-br-3.
I use RPMI-1640, 5% FBS, 1% penicillin, streptomycin and incubator: degree 37, 5% CO2
When i see microscope, circles in cells. I think this is different from autophagic vacuoles.
Is that contaminated bacteria or yeast?