Is that contaminated in cell culture(on breast cancer cell sk-br-3)?

Hi, everyone. first, i'm so sorry about i can't use English well...:( I research cancer cells and i culture breast cancel cell Sk-br-3. I use RPMI-1640, 5% FBS, 1% penicillin, streptomycin and...

11 February 2020 5,827 3 View

What kind of error in MTT assay?

I do MTT assay for cell viability. I used MTT solution 1mg/ml, 2h incubating and then treated DMSO to formazan. I read 595nm. In march, O.D was fine ( repeated 6 times ). So i perform...

04 July 2019 6,982 4 View

Why am i not able to detect LC3 band?

I try to do Western blot for LC3 band, but i can only see just one band. I do 16% running gel 90V 6h, 25V 1.5h to transfer, and i use primary antibody - Cell Signaling antibody #4108, then HRP...

02 March 2019 9,560 8 View

Is it normal AGS cells in flask?

Is it normal morpholagy AGS cells? The dark dot is bacteria. it was contaminated..but i have a question. I culture AGS cells in 75t falsk. It was filled about 70% on the floor, so i washed PBS and...

08 September 2018 3,486 1 View

Is it normal AGS cells in flask?

Is it normal morpholagy AGS cells? The dark dot is bacteria. it was contaminated..but i have a question. I culture AGS cells in 75t falsk. It was filled about 70% on the floor, so i washed PBS and...

02 September 2018 2,645 1 View

Cells were disappeard in 75t-flask just 1 day. Is that possible?

Hi, I culture AGS cells human gastric cancer cells. I use materials 'flavonoid' for inhibiting cancer cells. I use DMSO for solvent, 0.5% of total volume in RPMI. Anyway, i treated with various...

29 August 2018 245 8 View

This product cen be used detecting AVOs in autophagy?

Hi, i want to use acridine orange for dedecting autophagy. So, i find sigma-aldrich Acridine Orange hemi(zinc chloride) salt A6014. Conclusionsly, any people were used this product? I can't speak...

20 August 2018 9,215 2 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

Is there any rich, publicly available English literary corpus?

Hi everyone. I am looking for a rich corpus in English literature, a one which includes literary prose ( e.g., novels or short stories) . can anyone propose a good one? Best regards Fatemeh

01 March 2021 1,970 2 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

What are the saturated salt solutions that can give specific relative humidity of 35%, 65% and 95% for conditioning wood specimens?

Due to the unavailability of climate rooms, I want to carry out an experiment that require the use of certain saturated salt solutions especially those that can condition the wood specimen to 35%,...

25 February 2021 8,139 1 View

It is necessary to reflux the Schiff base and metal salts to obtain metal complex??

24 February 2021 9,166 3 View

How can I remove deep eutectic solvents from the medium?

Dear researchers, How can I remove deep eutectic solvents from the medium? You know, generally, deep eutectic solvents consist of ammonium salts and an acid group. Is there any alternative method...

22 February 2021 3,275 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View