Is that contaminated in cell culture(on breast cancer cell sk-br-3)?

Hi, everyone. first, i'm so sorry about i can't use English well...:( I research cancer cells and i culture breast cancel cell Sk-br-3. I use RPMI-1640, 5% FBS, 1% penicillin, streptomycin and...

11 February 2020 5,827 3 View

What kind of error in MTT assay?

I do MTT assay for cell viability. I used MTT solution 1mg/ml, 2h incubating and then treated DMSO to formazan. I read 595nm. In march, O.D was fine ( repeated 6 times ). So i perform...

04 July 2019 6,982 4 View

Why am i not able to detect LC3 band?

I try to do Western blot for LC3 band, but i can only see just one band. I do 16% running gel 90V 6h, 25V 1.5h to transfer, and i use primary antibody - Cell Signaling antibody #4108, then HRP...

02 March 2019 9,560 8 View

Is it normal AGS cells in flask?

Is it normal morpholagy AGS cells? The dark dot is bacteria. it was contaminated..but i have a question. I culture AGS cells in 75t falsk. It was filled about 70% on the floor, so i washed PBS and...

08 September 2018 3,486 1 View

Cells were disappeard in 75t-flask just 1 day. Is that possible?

Hi, I culture AGS cells human gastric cancer cells. I use materials 'flavonoid' for inhibiting cancer cells. I use DMSO for solvent, 0.5% of total volume in RPMI. Anyway, i treated with various...

29 August 2018 245 8 View

This product cen be used detecting AVOs in autophagy?

Hi, i want to use acridine orange for dedecting autophagy. So, i find sigma-aldrich Acridine Orange hemi(zinc chloride) salt A6014. Conclusionsly, any people were used this product? I can't speak...

20 August 2018 9,215 2 View

This product cen be used detecting AVOs in autophagy?

Hi, i want to use acridine orange for dedecting autophagy. So, i find sigma-aldrich Acridine Orange hemi(zinc chloride) salt A6014. Conclusionsly, any people were used this product? I can't speak...

07 August 2018 1,767 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View