8 Questions 11 Answers 0 Followers
Questions related from Jaehan Lee
Hi, everyone. first, i'm so sorry about i can't use English well...:( I research cancer cells and i culture breast cancel cell Sk-br-3. I use RPMI-1640, 5% FBS, 1% penicillin, streptomycin and...
12 February 2020 5,893 3 View
I do MTT assay for cell viability. I used MTT solution 1mg/ml, 2h incubating and then treated DMSO to formazan. I read 595nm. In march, O.D was fine ( repeated 6 times ). So i perform...
05 July 2019 7,043 4 View
I try to do Western blot for LC3 band, but i can only see just one band. I do 16% running gel 90V 6h, 25V 1.5h to transfer, and i use primary antibody - Cell Signaling antibody #4108, then HRP...
03 March 2019 9,617 8 View
Is it normal morpholagy AGS cells? The dark dot is bacteria. it was contaminated..but i have a question. I culture AGS cells in 75t falsk. It was filled about 70% on the floor, so i washed PBS and...
09 September 2018 3,544 1 View
03 September 2018 2,700 1 View
Hi, I culture AGS cells human gastric cancer cells. I use materials 'flavonoid' for inhibiting cancer cells. I use DMSO for solvent, 0.5% of total volume in RPMI. Anyway, i treated with various...
30 August 2018 310 8 View
Hi, i want to use acridine orange for dedecting autophagy. So, i find sigma-aldrich Acridine Orange hemi(zinc chloride) salt A6014. Conclusionsly, any people were used this product? I can't speak...
21 August 2018 9,280 2 View
08 August 2018 1,833 3 View
