11 July 2024 2 6K Report

How much PVP-K30 should be used as alternative to PVP-40 in DNA isolation.

More Shivansh Negi's questions See All
Is it necessary to autoclave every vessel used in DNA isolation?

DNA isolation requires many freshly made buffers. So is it necessary to autoclave each vessel used, before preparing buffers in them?

10 July 2024 7,526 3 View

What are the common mistakes occur during DNA isolation in plants?

General mistakes which could happen during DNA isolation of plants

10 July 2024 9,488 3 View

Where can I find a reliable(peer reviewed) source code for the QKD BB84 protocol?

I'm trying to implement BB84 on a network, however I don't have a source code that is backed by any organization or a peer reviewed paper. Any help would be appreciated. Thanks!

09 June 2024 5,786 1 View

If I have 1000 or more plant extracts, which is the best latest technology for the rapid and highly accurate identification of secondary metabolites?

the query centers on determining the most efficient and precise latest technology available in the market, capable of swiftly analyzing complex mixtures while maintaining a high level of...

26 May 2024 2,898 6 View

Enzyme is losing activity after 24 hours. what could be the reason?

I'm working on enzyme beta glucosidase. I used CMC in my production media and the activity starts coming after 5 hours but after 24 hrs no activity is coming. Has anybody experienced the same problem?

24 March 2023 8,277 2 View

Why is Beta glucosidase enzyme is losing activity after urea treatment?

I've clone a gene of Beta glucosidase enzyme in E.coli. Most of my protein is going in inclusion bodies. So I tried the urea treatment for the solubilization of inclusion bodies and afterwards...

09 March 2023 2,667 2 View

How nanofluid can be used for solar distillation. When we pour nano fluid inside the basin some water will evaporate that decrease the fluid level?

Water will evaporate and remaining nano particles will be settled down so how can we avoid that

02 February 2023 9,969 1 View

Is Medicon Open Access SCI Indexed?

I have received an email regarding publishing in Medicon Open Access Journals. Upon checking two particular journals had an Impact Factor of 1.2 average. However, upon checking both Clarivate and...

01 February 2023 137 6 View

Can we use biorender.com for creating images for publication?

So, I used biorender paid subscription for creating images but my prof fear that good journals like cell or nature may not accept images created using biorender since other researchers too might...

31 January 2023 9,076 0 View

Solvent for oil sample dilution.?

Which solvent should be used to dilute plant oil samples extracted through hydrodistilation process. I used DMSO and Hexane solvent for dilution but oil sample got pricipitated in the Hexane and...

21 December 2022 5,207 1 View

Similar questions and discussions
Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close