Shivansh Negi

10 Questions 8 Answers 0 Followers

Questions related from Shivansh Negi

I treated genomic DNA sample with RNAse A. But I did not get distinct bands. What could be the possible reasons?

I got smeared bands after the isolation of genomic DNA from a plant. I treated the samples with RNAse A, but again smeared bands appeared, though less visible (Media attached). What could be the...

08 October 2024 2,227 4 View

I observed smeared DNA bands with glowy wells in gel electrophoresis (Media attached). What could be the reason and solutions?

I ran gel electrophoresis of DNA samples, got glowy wells, and smeared DNA bands. Though the primary reason is contamination, I want to know the specific reasons and solutions.

23 September 2024 791 6 View

I am getting a smeared fainted band in the gel after gel electrophoresis of my DNA samples. What it could be?

What is the reason of smeared fainted band in gel electrophoresis of DNA.

03 September 2024 7,182 3 View

I added wash buffer to the pellet after DNA isolation. I am confused is this DNA or not?

DNA pellet in wash buffer seems different in my experience. Is this a DNA molecule or something else?

19 July 2024 833 5 View

What are the common mistakes occur during DNA isolation in plants?

General mistakes which could happen during DNA isolation of plants

11 July 2024 9,528 3 View

Is it necessary to autoclave every vessel used in DNA isolation?

DNA isolation requires many freshly made buffers. So is it necessary to autoclave each vessel used, before preparing buffers in them?

11 July 2024 7,607 3 View

What is the alternative for liquid nitrogen in DNA isolation?

Many protocols mention the use of liquid nitrogen for grinding plant tissues, is there any economical alternative to this which works well

11 July 2024 947 6 View

What is the alternative to ammoniium acetate mention in DNA isolation protocol of Doyle &Doyle, 1991?

In the Doyle and Doyle, 1991 protocol for plant DNA isolation, requirement of ammonium acetate is mentioned. Is there any alternative to it?

11 July 2024 1,209 1 View

Is PVP-K30 alternative to PVP-40 used in DNA isolation?

How much PVP-K30 should be used as alternative to PVP-40 in DNA isolation.

11 July 2024 6,086 2 View

While preparing buffers for DNA isolation, the salts usually remain unmixed in the solution, though weighed accurately. Why? What's the solution?

Salts usually remain unmixed in the solutions, even after vigorous mixing, while preparing buffers for DNA isolation. Is there a method to it?

01 January 1970 8,460 1 View