Is Olig1 expressed in any non-oligodendroglial lineage cells (NSC, OPC, oligodendrocyte)?

More Stuart Iain Jenkins's questions See All

Is immunostaining for macroH2A.1 a good way to differentiate male and female (sex, gender) rat cells in co-culture? Stains only female cells?

I want to identify individual male and female rat cells in co-culture (microglia, astrocytes). Ideally, want technique compatible with further immunostaining. In situ hybridisation seems overly...

08 September 2015 8,755 1 View

Can antibodies / immunoglobulins cross the intact blood-brain barrier (BBB) in 'healthy' humans / rodents?

Antibodies are found in the central nervous system in disease/injury states (e.g. multiple sclerosis). But, do immunoglobulins cross the blood-brain barrier independently, or do invading plasma...

02 March 2015 1,049 5 View

Which microglia genes are appropriate for qPCR or RT-PCR normalisation in cells challenged with LPS (lipopolysaccharide/endotoxin)?

I am investigating changes in microglial gene expression, using qPCR (real time, reverse transcription). Microglia will be challenged with LPS while I establish the protocol, then various...

01 February 2015 9,076 10 View

Are there any standardised methods or general principles for validating in vitro assays as being representative models of in vivo?

I'm trying to assess the utility of various in vitro assays/models, and I'm looking for *general thoughts/opinions* here. Elsewhere, I'm looking at the specifics of modelling the brain, modelling...

07 August 2013 977 1 View

Is anybody using Alvetex 3D cell culture scaffolds? What are your experiences?

Alvetex is a porous polystyrene block in which cells can be cultured. Cells are purported to adopt more biologically relevant morphologies and behaviours. I like the theory, but has anybody tried...

02 March 2013 3,624 1 View

Are there petri dishes which are less than 10 mm in height available?

I need a petri dish, 60 mm diameter or less, with height less than 10 mm. I need to use an upright microscope to image the samples in a petri dish (ideally 60 or 35 mm diameter), but I have an...

02 March 2013 8,579 8 View

Does anyone have any experience of PEG-coated fluorescent magnetic particles or nanoparticles? Specifically, cellular uptake of such particles?

I want to label cells with fluorescent particles, and study the influence of various coatings/surface properties on extent of uptake. I need a commercially available polyethylene glycol...

11 December 2012 3,638 6 View

iZON qNano particle analyser - is anyone using this device?

Does anyone have any experience of using this device (qNano) to characterise nanoparticles? In particular, I'm interested in characterising iron oxide-based particles, with surfaces including...

09 October 2012 3,444 8 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

What should Berlin do as a city to become as impactful as London and Paris in World Football?

Please go through my Abstract. I can also share a proposed Thesis Outline.

04 August 2024 2,077 0 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

What are the roles of innovation in achieving the Sustainable Development Goals (SDG)?

31 July 2024 3,533 2 View

How to find projects for a team of professional developers in AI?

We are looking for projects for a team of professional developers About us We are a team of experienced developers with over 10 years of experience in various programming fields. Our mission is to...

30 July 2024 8,377 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Technology developments may it for whatever reasons developed eventually its benefits are multi-Disciplinatary?

Your comments

28 July 2024 2,822 2 View

Any direct measurement to measure the bacteria or micro organism using IOT or any indirect measurement?

need of sensor details or sensor development for this question.

28 July 2024 4,539 3 View

Jorge Moreno

As far as I recall Olig genes are already expresed in embryonic oligodendroglial precursors and also in the mature oligodendrocytes. Olig1, specifically is related tot he promotion of an chondroitin sulfate proteoglycan+ glial cell progenitor. Hope this helps!

Stuart Iain Jenkins

I've seen the suggestion that Olig1 is expressed in liver, kidney and lung, and in monocytes.

Can anyone confirm this?

Does anyone know what functions Olig1 may perform in non-oligodendrocytes?

Thank you, Josh, but what I'm looking for is evidence of Olig1 expression/function in non-oligodendroglial lineage cells.