Is immunostaining for macroH2A.1 a good way to differentiate male and female (sex, gender) rat cells in co-culture? Stains only female cells?

I want to identify individual male and female rat cells in co-culture (microglia, astrocytes). Ideally, want technique compatible with further immunostaining. In situ hybridisation seems overly...

08 September 2015 8,621 1 View

Can antibodies / immunoglobulins cross the intact blood-brain barrier (BBB) in 'healthy' humans / rodents?

Antibodies are found in the central nervous system in disease/injury states (e.g. multiple sclerosis). But, do immunoglobulins cross the blood-brain barrier independently, or do invading plasma...

02 March 2015 938 5 View

Which microglia genes are appropriate for qPCR or RT-PCR normalisation in cells challenged with LPS (lipopolysaccharide/endotoxin)?

I am investigating changes in microglial gene expression, using qPCR (real time, reverse transcription). Microglia will be challenged with LPS while I establish the protocol, then various...

01 February 2015 8,919 10 View

Are there any standardised methods or general principles for validating in vitro assays as being representative models of in vivo?

I'm trying to assess the utility of various in vitro assays/models, and I'm looking for *general thoughts/opinions* here. Elsewhere, I'm looking at the specifics of modelling the brain, modelling...

07 August 2013 870 1 View

Are there petri dishes which are less than 10 mm in height available?

I need a petri dish, 60 mm diameter or less, with height less than 10 mm. I need to use an upright microscope to image the samples in a petri dish (ideally 60 or 35 mm diameter), but I have an...

02 March 2013 8,468 8 View

Is anybody using Alvetex 3D cell culture scaffolds? What are your experiences?

Alvetex is a porous polystyrene block in which cells can be cultured. Cells are purported to adopt more biologically relevant morphologies and behaviours. I like the theory, but has anybody tried...

02 March 2013 3,482 1 View

Does anyone have any experience of PEG-coated fluorescent magnetic particles or nanoparticles? Specifically, cellular uptake of such particles?

I want to label cells with fluorescent particles, and study the influence of various coatings/surface properties on extent of uptake. I need a commercially available polyethylene glycol...

11 December 2012 3,505 6 View

iZON qNano particle analyser - is anyone using this device?

Does anyone have any experience of using this device (qNano) to characterise nanoparticles? In particular, I'm interested in characterising iron oxide-based particles, with surfaces including...

09 October 2012 3,308 8 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

No title

01 March 2021 3,905 5 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How to sample data to increase presence of solar panels in the images?

I am working on creating deep neural network on geospatial dataset and wanted to know how to do the sampling so that I can increase the presence of solar panels in the images. I cannot use entire...

25 February 2021 3,936 6 View