Dear Stuart, I have used both ChemiCell and Micromod fluorescent MNPs. These low-toxicity PEG-coated MNPs did pretty well on our experiments. Currently however, we make our own magnetite PEI-Alexa-Magnetic nanoparticles. Please note that in vitro toxicity is not related to in vivo toxicity.There is abundant literature about uptake dependence on NP coating, and of course the results are strongly dependent on the cell line studied.

regards

Gerardo.

Yes see our old publication

We are using magnetite nanoparticles coated with various PEI derivatives (that we make) for transfecting genes and siRNA’s to a variety of human cell lines. I understand the PEG modified NP’s should work well too. But as mentioned before, there are many reports on the relationship between surface properties and cellular uptake (and toxicity). Keep in mind that different cell lines behave differently, and in vivo it’s a whole different game. Also, if your intention is to level specific cells, I think you might have to consider the time these particles remain in the cytoplasm. Best of luck.

Gabriel.

Sorry ignore the file given one hour before, it was a wrong file. Here the correct one.

We use Peg-coated liposomes for gene delivery which we synthesize.Theoretically, pegylation reduces opsonization of nanoparticles which alters its cellular biodistribution. There does exist a relationship between surface characteristics of your delivery vehicle and how its interacts with a cell or endocytic uptake. These relationships are heavily influence by cell type and in vitro situations (cytotoxicity, transfection) does not necessarily translate into in vivo success.

Kovashnee Naicker

An alternative coating by polypyrrole and effects by this coating, probably it will be interesting and on some extent similar to PEG coatings:

Article Stabilization of (CdSe)ZnS Quantum Dots with Polypyrrole For...