Can antibodies / immunoglobulins cross the intact blood-brain barrier (BBB) in 'healthy' humans / rodents?

Antibodies are found in the central nervous system in disease/injury states (e.g. multiple sclerosis). But, do immunoglobulins cross the blood-brain barrier independently, or do invading plasma...

02 March 2015 938 5 View

Which microglia genes are appropriate for qPCR or RT-PCR normalisation in cells challenged with LPS (lipopolysaccharide/endotoxin)?

I am investigating changes in microglial gene expression, using qPCR (real time, reverse transcription). Microglia will be challenged with LPS while I establish the protocol, then various...

01 February 2015 8,919 10 View

Are there any standardised methods or general principles for validating in vitro assays as being representative models of in vivo?

I'm trying to assess the utility of various in vitro assays/models, and I'm looking for *general thoughts/opinions* here. Elsewhere, I'm looking at the specifics of modelling the brain, modelling...

07 August 2013 870 1 View

Are there petri dishes which are less than 10 mm in height available?

I need a petri dish, 60 mm diameter or less, with height less than 10 mm. I need to use an upright microscope to image the samples in a petri dish (ideally 60 or 35 mm diameter), but I have an...

02 March 2013 8,468 8 View

Is anybody using Alvetex 3D cell culture scaffolds? What are your experiences?

Alvetex is a porous polystyrene block in which cells can be cultured. Cells are purported to adopt more biologically relevant morphologies and behaviours. I like the theory, but has anybody tried...

02 March 2013 3,482 1 View

Is Olig1 expressed in any non-oligodendroglial lineage cells (NSC, OPC, oligodendrocyte)?

Furthermore, could such expression in other cell types affect oligodendrocyte precursors (OPCs) through some downstream pathway?

31 December 2012 8,910 3 View

Does anyone have any experience of PEG-coated fluorescent magnetic particles or nanoparticles? Specifically, cellular uptake of such particles?

I want to label cells with fluorescent particles, and study the influence of various coatings/surface properties on extent of uptake. I need a commercially available polyethylene glycol...

11 December 2012 3,505 6 View

iZON qNano particle analyser - is anyone using this device?

Does anyone have any experience of using this device (qNano) to characterise nanoparticles? In particular, I'm interested in characterising iron oxide-based particles, with surfaces including...

09 October 2012 3,308 8 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

No title

02 March 2021 3,060 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View