Is immunostaining for macroH2A.1 a good way to differentiate male and female (sex, gender) rat cells in co-culture? Stains only female cells?

Stuart Iain Jenkins @Stuart_Jenkins

09 September 2015 1 9K Report

I want to identify individual male and female rat cells in co-culture (microglia, astrocytes).

Ideally, want technique compatible with further immunostaining.

In situ hybridisation seems overly complex to setup.

Barr body identification seems

Homa Adle-Biassette

Hi Stuart

I only have experience with ISH combined with immunohistochemistry, tested to detect the origin of the lymphoma cells in transplanted patients having a difference gender.

Several protocols exists.

Good luck

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