Does anyone have any experience of using this device (qNano) to characterise nanoparticles?
In particular, I'm interested in characterising iron oxide-based particles, with surfaces including polystyrene, fluorophores, PEI, antibodies, etc.
Has anyone successfully measured diameter, charge (zeta potential), concentration of such particles?
Thank you Eugen.
What did you think of the demo?
Did it provide both size and charge data?
Did you get to run any samples yourself?
Can I ask roughly what the NanoSight device costs?
Thanks,
Stuart
I have just begun my Level 1 training on the IZON qNano. As a student my access is limited, however, this instrument has the ability to measure all characteristics you listed above. It is a remarkable technology that reduces uncertainty that may be observed with other characterization instuments.
Hi Nadiah.
I'm interested in characterising iron oxide nanoparticles, used to label and/or transfect cells. The particles I'm using are ~35 nm, ~150 nm and ~360 nm, with varying coatings. Some are cationic, some anionic.
As some are transfection-grade, I'd like to know how large they are once functionalised with nucleic acids, and how this affects their charge.
Is the charge/zeta potential and average value? Presumably the charge can vary across the surface of the particle?
Will the size be determined in 1 axis? That is, would a cylinder be measured in whatever orientation it passed through the sensor?
What happens when particles aggregate? Do small aggregations pass through the sensor, and get measured, while large aggregations remain in the sample chamber?
Thanks,
Stuart
hi Nadia, is a hand held version that gives surface charge available? i.e. something that i can use during real time emission assessment
We just purchased this device for the analysis of biological particles. It is less user friendly than the newest Nanosight platforms, but about 1/3rd of the cost.
You can read this article
http://www.sciencedirect.com/science/article/pii/S0021979713001756
Is anyone using iZON qNano for zeta analyser?
I need to measure the zeta potential of my liposome samples and I need to know how to operate iZON qNano and how to prepare my sample?
Thank you
Yes, several detailed studies of liposome charge have been done, including distinguishing mixtures of PS and PC lipids in varying concentrations. Particle by particle charge analysis enables the researcher to see precisely what is happening in their samples. In that case varying the mixtures showed how the PS and PC interacted.
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