Can I use lung organoid for cystic fibrosis research?
At least you could try. 3D cultures usually mimick the real microenvironment much better than cells growing in monolayer.
Quite nice publication about differences between 2Dand 3D: Article Modeling Physiological Events in 2D vs. 3D Cell Culture
Thank you Vilma Petrikaite
In regard of cystic fibrosis research.
11 December 2018 5,355 2 View
What is the biology of in vitro mRNA production from amplified DNA by PCR?
11 December 2018 5,043 6 View
Currently, I have about 70 Staphylococcus aureus samples, and I want to find out their specific strains.
02 March 2018 3,773 4 View
I'm doing this project to identify MRSA. In my antibiogram there are some samples that are showing resistance to oxacillin but not in cefoxitin. What might be the interpretation? Is it because...
08 September 2017 4,393 3 View
staphylococcus aureus have been cultured on Mannitol Salt Agar (MSA).
07 August 2017 1,835 6 View
I will be needing those antibodies for CD34, GPC3, and s-FRP4.
10 November 2016 7,931 0 View
I will be using blood samples. So, Is it appropriate to use ELISA or should I use western blotting, followed by densitometric analysis to determine protein level?
10 November 2016 1,786 15 View
I will be using ELISA for detection of protein.
10 November 2016 2,654 5 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...
05 August 2024 5,829 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View