What are established cell lines used in lung research?

In regard of cystic fibrosis research.

11 December 2018 5,307 2 View

How to produce synthetic mRNA?

What is the biology of in vitro mRNA production from amplified DNA by PCR?

11 December 2018 4,963 6 View

How much does it cost to do PCR? is 16rna sequencing cheaper than PCR?

Currently, I have about 70 Staphylococcus aureus samples, and I want to find out their specific strains.

02 March 2018 3,714 4 View

What is difference between heteroresistance and intermediate resistance?

I'm doing this project to identify MRSA. In my antibiogram there are some samples that are showing resistance to oxacillin but not in cefoxitin. What might be the interpretation? Is it because...

08 September 2017 4,337 3 View

How long can I store staphylococcus aureus in fridge?

07 August 2017 1,716 6 View

Is there any indian or asian biotech company manufactures primary and secondary antibodies for ELISA?

10 November 2016 7,882 0 View

Can I detect CD34 protein in serum in hepatocellular carcinoma?

10 November 2016 2,579 5 View

How to determine protein concentration level in blood?

I will be using blood samples. So, Is it appropriate to use ELISA or should I use western blotting, followed by densitometric analysis to determine protein level?

10 November 2016 1,730 15 View

No title

02 March 2021 3,060 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View