What are established cell lines used in lung research?

In regard of cystic fibrosis research.

11 December 2018 5,307 2 View

Is lung organoid better than 2D cell culture?

Can I use lung organoid for cystic fibrosis research?

11 December 2018 7,784 2 View

How to produce synthetic mRNA?

What is the biology of in vitro mRNA production from amplified DNA by PCR?

11 December 2018 4,963 6 View

How much does it cost to do PCR? is 16rna sequencing cheaper than PCR?

Currently, I have about 70 Staphylococcus aureus samples, and I want to find out their specific strains.

02 March 2018 3,714 4 View

What is difference between heteroresistance and intermediate resistance?

I'm doing this project to identify MRSA. In my antibiogram there are some samples that are showing resistance to oxacillin but not in cefoxitin. What might be the interpretation? Is it because...

08 September 2017 4,337 3 View

Is there any indian or asian biotech company manufactures primary and secondary antibodies for ELISA?

10 November 2016 7,882 0 View

Can I detect CD34 protein in serum in hepatocellular carcinoma?

10 November 2016 2,579 5 View

How to determine protein concentration level in blood?

I will be using blood samples. So, Is it appropriate to use ELISA or should I use western blotting, followed by densitometric analysis to determine protein level?

10 November 2016 1,730 15 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

What are the saturated salt solutions that can give specific relative humidity of 35%, 65% and 95% for conditioning wood specimens?

Due to the unavailability of climate rooms, I want to carry out an experiment that require the use of certain saturated salt solutions especially those that can condition the wood specimen to 35%,...

25 February 2021 8,139 1 View

It is necessary to reflux the Schiff base and metal salts to obtain metal complex??

24 February 2021 9,166 3 View

How can I remove deep eutectic solvents from the medium?

Dear researchers, How can I remove deep eutectic solvents from the medium? You know, generally, deep eutectic solvents consist of ammonium salts and an acid group. Is there any alternative method...

22 February 2021 3,275 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View