What are established cell lines used in lung research?

In regard of cystic fibrosis research.

11 December 2018 5,307 2 View

Is lung organoid better than 2D cell culture?

Can I use lung organoid for cystic fibrosis research?

11 December 2018 7,784 2 View

How to produce synthetic mRNA?

What is the biology of in vitro mRNA production from amplified DNA by PCR?

11 December 2018 4,963 6 View

What is difference between heteroresistance and intermediate resistance?

I'm doing this project to identify MRSA. In my antibiogram there are some samples that are showing resistance to oxacillin but not in cefoxitin. What might be the interpretation? Is it because...

08 September 2017 4,337 3 View

How long can I store staphylococcus aureus in fridge?

07 August 2017 1,716 6 View

Is there any indian or asian biotech company manufactures primary and secondary antibodies for ELISA?

10 November 2016 7,882 0 View

Can I detect CD34 protein in serum in hepatocellular carcinoma?

10 November 2016 2,579 5 View

How to determine protein concentration level in blood?

I will be using blood samples. So, Is it appropriate to use ELISA or should I use western blotting, followed by densitometric analysis to determine protein level?

10 November 2016 1,730 15 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View