How much does it cost to do PCR? is 16rna sequencing cheaper than PCR?

More Sumit Biswas's questions See All

What are established cell lines used in lung research?

In regard of cystic fibrosis research.

11 December 2018 5,355 2 View

Is lung organoid better than 2D cell culture?

Can I use lung organoid for cystic fibrosis research?

11 December 2018 7,841 2 View

How to produce synthetic mRNA?

What is the biology of in vitro mRNA production from amplified DNA by PCR?

11 December 2018 5,043 6 View

What is difference between heteroresistance and intermediate resistance?

I'm doing this project to identify MRSA. In my antibiogram there are some samples that are showing resistance to oxacillin but not in cefoxitin. What might be the interpretation? Is it because...

08 September 2017 4,393 3 View

How long can I store staphylococcus aureus in fridge?

staphylococcus aureus have been cultured on Mannitol Salt Agar (MSA).

07 August 2017 1,835 6 View

Is there any indian or asian biotech company manufactures primary and secondary antibodies for ELISA?

I will be needing those antibodies for CD34, GPC3, and s-FRP4.

10 November 2016 7,931 0 View

How to determine protein concentration level in blood?

I will be using blood samples. So, Is it appropriate to use ELISA or should I use western blotting, followed by densitometric analysis to determine protein level?

10 November 2016 1,786 15 View

Can I detect CD34 protein in serum in hepatocellular carcinoma?

I will be using ELISA for detection of protein.

10 November 2016 2,654 5 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Identify the eight key processes of excellent supply chain management and discuss how each of these processes impacts the end customer?

Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26

04 August 2024 9,919 4 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Mohammed H. Musleh Popular answer

Search the net and you will find thousands of references!!

just one here

Article Genetic Identification of Staphylococcus aureus by Polymeras...

Mushtak T. S. Al-Ouqaili

Then you have to do sequencing!! Just PCR will not be helpful!!

Cost issue you may figure it out via web, try to find suitable offers.

Best luck.

Badreddine Mechri

Hi Sumit,

First you should amplify the 16S rRNA gene using universal primers, then you sequence the PCR product. Good Luck.

Mohammed H. Musleh