What are established cell lines used in lung research?

More Sumit Biswas's questions See All

Is lung organoid better than 2D cell culture?

Can I use lung organoid for cystic fibrosis research?

11 December 2018 7,841 2 View

How to produce synthetic mRNA?

What is the biology of in vitro mRNA production from amplified DNA by PCR?

11 December 2018 5,043 6 View

How much does it cost to do PCR? is 16rna sequencing cheaper than PCR?

Currently, I have about 70 Staphylococcus aureus samples, and I want to find out their specific strains.

02 March 2018 3,773 4 View

What is difference between heteroresistance and intermediate resistance?

I'm doing this project to identify MRSA. In my antibiogram there are some samples that are showing resistance to oxacillin but not in cefoxitin. What might be the interpretation? Is it because...

08 September 2017 4,393 3 View

How long can I store staphylococcus aureus in fridge?

staphylococcus aureus have been cultured on Mannitol Salt Agar (MSA).

07 August 2017 1,835 6 View

Is there any indian or asian biotech company manufactures primary and secondary antibodies for ELISA?

I will be needing those antibodies for CD34, GPC3, and s-FRP4.

10 November 2016 7,931 0 View

How to determine protein concentration level in blood?

I will be using blood samples. So, Is it appropriate to use ELISA or should I use western blotting, followed by densitometric analysis to determine protein level?

10 November 2016 1,786 15 View

Can I detect CD34 protein in serum in hepatocellular carcinoma?

I will be using ELISA for detection of protein.

10 November 2016 2,654 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

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07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

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Amos Bairoch

Although:

https://www.ncbi.nlm.nih.gov/pubmed/?term=15463957

is a publication from 2004, it is still quite useful as a review of CF cell lines. The corresponding entries in the Cellosaurus can be found by doing the query:

https://web.expasy.org/cgi-bin/cellosaurus/search?input=15463957

Best

Amos

Sumit Biswas

Thank you.