Is liquid nitrogen compulsory for DNA virus extraction from plant samples?

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Shiva Kumar

Hlo Krishnamoorthy

Without using liquid N2, the leaves would not be grinded in a fine way. After the tissues are evenly grinded, DNA could be easily extracted. Added to that it will be free of contaminants too.

Hassan Nima

Is very necessary to ensure that the leaves are well and evenly grounded, preventing contamination

Janarthanan P.

I agree with Siva Kumar.

Mushtak T. S. Al-Ouqaili

Fine grinding can't be performed without liquid nitrogen!! This step is necessary for good DNA yield. I experienced CTAB as homogenizer followed by extraction buffer protocol, DNA was in excellent Quantity and Quality!

Best Luck.