I am doing a work constructing molecular clone (infectious clone) of virus and i wonder whether the length of the poly A tails will affects the replication of virus? i made it one with about 48 A's and one with 24 A's only, the aim of this work is to compare the replication kinetics of this two viruses, is it okay to compare with different length of poly A tails?
To my knowledge, the polyA tail would definitely interfere with the replication dynamics. apparently the shorter the better for many viruses
http://www.abstracts2view.com/asgct/view.php?nu=ASGCT14L1_114
RNA viruses typically have long terminal repeats (LTRs) at the ends of their genomes that are necessary for viral replication. You can't substitute polyA sequences for these, and in fact polyA sequences would prevent viral replication by causing improper termination of transcription prior to the LTR.
I am currently doing picornavirus works, from what i referred to those clones consists of a poly A tails instead of LTR.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Molecular Methods
- Cloning