Daniel M Cohen

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Questions related from Daniel M Cohen

Gene signature for "stemness" in human mesenchymal stem cells - can anyone help?

Is there a set of genes appropriate for profiling "stemness" in human mesenchymal stem cells? There are a handful of papers that have suggested possible stemness markers, such as "Identification...

10 June 2013 5,112 14 View

Are introns spliced out of lentiviral transcripts prior to packaging into virions?

Intronic sequences are often useful to promote gene expression, either due to beneficial effects of RNA splicing on mRNA stability, or due to the presence of intronic enhancers that boost...

30 May 2013 6,614 8 View

When does PolII occupancy at regulatory regions change independently of chromatin state?

Can anyone highlight a study or studies in which PolII occupancy at enhancers or promoters was shown to change independently of, or temporarily preceding, changes in epigenetic chromatin marks...

16 May 2013 2,701 9 View

Can anyone recommend a specific fluorescent reporter to assess promoter activity in a longitudinal study?

I am currently using EGFP fused to the PEST sequence of the mouse ornithine decarboxylase (MODC) gene. However, its response to modulating promoter activity is a bit sluggish (still better than WT...

15 February 2013 4,179 14 View